In situ hybridization to study the origin and fate of identified neurons.
In situ hybridization to study the origin and fate of identified neurons.
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原位杂交研究已识别神经元的起源和命运。
DOI：
10.1126/science.6356362
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Axel,R
中科院分区：
文献类型：
--
作者：
McAllister,LB;Scheller,RH;Kandel,ER;Axel,R
鸟类的产卵行为是由一组肽介导的，包括产卵激素（ELH），这些肽是由一群已识别的神经元（袋细胞）释放的。已经分离出一个神经肽基因家族，该家族包括编码ELH的基因沿着以及另外两个编码被认为启动产卵过程的A和B肽的基因，并且已经确定了它们的核苷酸序列。用原位杂交和免疫荧光技术研究表达该基因家族的神经元的起源和分布。ELH基因不仅在袋细胞中表达，而且在中枢神经系统的五个神经节中的四个中分布的广泛的神经元系统中表达。ELH的基因在动物生命周期的早期在这些细胞中表达。因此，有可能使用原位杂交来追踪表达ELH的细胞到它们的起源位点。这些细胞起源于体壁外胚层的中枢神经系统外，并通过沿着结缔组织链爬行而迁移到中枢神经系统内的最终位置。
Egg-laying behavior inAplysiais mediated by a set of peptides, including egg-laying hormone (ELH), which are released by a cluster of identified neurons, the bag cells. A family of neuropeptide genes which includes the gene encoding ELH along with two additional genes encoding the A and B peptides thought to initiate the egg-laying process has been isolated and their nucleotide sequence has been determined. In situ hybridization and immunofluorescence was used to explore the origin and distribution of the neurons that express this family of genes. The ELH genes are expressed, not only in the bag cells, but in an extensive system of neurons distributed in four of the five ganglia of the central nervous system. The genes for ELH are expressed in these cells early in the animal's life cycle. As a result, it was possible to use in situ hybridization to trace the cells expressing ELH to their site of origin. The cells originate outside the central nervous system in the ectoderm of the body wall and appear to migrate to their final locations within the central nervous system by crawling along strands of connective tissue.