beta-Conglycinin enhances autophagy in porcine enterocytes
beta-Conglycinin enhances autophagy in porcine enterocytes
复制标题
β-伴大豆球蛋白增强猪肠细胞的自噬
DOI：
10.1007/s00726-016-2352-7
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Wu Guoyao
中科院分区：
文献类型：
--
作者：
Yi Dan;Hou Yongqing;Mei Huimin;Wang Lei;Hu Chien-An Andy;Wu Guoyao
β-伴大豆球蛋白（β-CG）是引起新生猪和幼猪肠道过敏和功能障碍的重要物质。然而，其潜在的机制在很大程度上是未知的。为了阐明自噬在β-CG诱导的细胞毒性中的作用，我们研究了β-CG对猪肠上皮细胞（IPEC-1细胞）活力和自噬活性的影响。结果表明，随着β-CG浓度的增加，细胞活力下降。β-CG处理增强了eGFP-LC 3的细胞斑点数和LC 3-II/LC 3-I，并且后者在巴弗洛霉素A1培养的IPEC-1细胞中进一步增加。我们的结论是，β-CG增强肠上皮细胞的自噬活性。
β-Conglycinin (β-CG) is well known for inducing intestinal allergies and dysfunction in neonates and young pigs. However, the underlying mechanisms are largely unknown. In this study, to clarify the role of autophagy in β-CG-induced cytotoxicity, we investigated the effects of β-CG on cell viability and autophagy activity in porcine enterocytes (IPEC-1 cells). The results indicated that the cell viability was decreased with the increasing levels of β-CG. β-CG treatment enhanced the eGFP-LC3 puncta per cells and LC3-II/LC3-I, and the latter was further increased in IPEC-1 cells cultured with bafilomycin A1. We conclude that β-CG enhances autophagy activity in enterocytes.