Effect of high fat corn oil, olive oil and fish oil on phospholipid fatty acid composition in male F344 rats.
Effect of high fat corn oil, olive oil and fish oil on phospholipid fatty acid composition in male F344 rats.
复制标题
高脂玉米油、橄榄油和鱼油对雄性F344大鼠磷脂脂肪酸组成的影响。
作者：
Rao,CV;Zang,E;Reddy,BS
流行病学和实验动物模型研究提供的证据表明，膳食脂肪对结肠肿瘤发生的影响取决于脂肪的量及其成分。由于膳食脂肪组成和组织膜脂肪酸组成在肿瘤促进中的重要性，因此设计了实验来研究富含ω3、ω6和ω9脂肪酸的高脂饮食和结肠致癌物对肝脏、结肠、小肠、红细胞和血浆的磷脂脂肪酸组成的相对影响。 6周龄时，各组动物饲喂含有5%玉米油(LFCO)、23.5%玉米油(HFCO)、23.5%橄榄油(HFOO)和20.5%鱼油加3%玉米油(HFFO)的饮食。两周后，除媒介物处理的动物外，所有动物以15mg/kg体重的剂量率每周一次接受氧化偶氮甲烷，持续2周。 5天后处死动物，分析肝脏、结肠、小肠和红细胞以及血浆中的磷脂脂肪酸。结果表明，HFCO 饮食喂养的动物肝脏、结肠和小肠的磷脂脂肪酸组成与 LFCO 饮食喂养的动物没有显着差异。与饲喂LFCO饮食的动物相比，饲喂HFCO饮食的动物的红细胞和血浆中棕榈油酸和亚油酸的水平增加。与HFCO饮食相比，饲喂HFCO饮食显着增加了各器官中的油酸含量并降低了亚油酸和花生四烯酸水平。与饲喂HFCO饮食的动物相比，饲喂HFFO饮食的动物的二十碳五烯酸和二十二碳六烯酸显着增加，而亚油酸和花生四烯酸水平显着降低。结果还表明，致癌剂处理对磷脂脂肪酸组成的影响极小。
Epidemiological and laboratory animal model studies have provided evidence that the effect of dietary fat on colon tumorigenesis depends on the amount of fat and its composition. Because of the importance of the composition of dietary fat and of tissue membrane fatty acid composition in tumor promotion, experiments were designed to investigate the relative effects of high fat diets rich in ω3, ω6 and ω9 fatty acids and colon carcinogen on the phospholipid fatty acid composition of liver, colon, small intestine, erythrocytes and blood plasma. At 6 wk of age, groups of animals were fed diets containing 5% corn oil (LFCO), 23.5% corn oil (HFCO), 23.5% olive oil (HFOO), and 20.5% fish oil plus 3% corn oil (HFFO). Two weeks later all the animals except the vehicle-treated animals received azoxymethanes.c.once weekly for 2 wk at a dose rate of 15 mg/kg body weight. Animals were sacrificed 5 d later and liver, colon, small intestine and erythrocytes and blood plasma were analyzed for phospholipid fatty acids. The results indicate that the phospholipid fatty acid composition of liver, colon and small intestine of HFCO diet fed animals, were not significantly different from those fed the LFCO diet. The levels of palmitoleic acid and linoleic acid were increased in erythrocytes and blood plasma of the animals fed the HFCO diet compared to those fed the LFCO diet. Feeding the HFCO diet significantly increased the oleic acid content and decreased the linoleic acid and arachidonic acid levels in various organs when compared to the HFCO diet. Animals fed the HFFO diet showed a marked increase in eicosapentaenoic acid and docosahexaenoic acid and a decrease in linoleic acid and arachidonic acid levels as compared to those fed the HFCO diet. The results also indicate that carcinogen treatment had only a minimal effect on the phospholipid fatty acid composition.