SEQUENCE-ANALYSIS SUGGESTS THAT TETRA-NUCLEOTIDES SIGNAL THE TERMINATION OF PROTEIN-SYNTHESIS IN EUKARYOTES
SEQUENCE-ANALYSIS SUGGESTS THAT TETRA-NUCLEOTIDES SIGNAL THE TERMINATION OF PROTEIN-SYNTHESIS IN EUKARYOTES
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DOI：
10.1093/nar/18.21.6339
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发表时间：
1990-11-11
影响因子：
14.9
通讯作者：
TATE, WP
中科院分区：
文献类型：
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作者：
BROWN, CM;STOCKWELL, PA;TATE, WP
越来越多的情况下，三核苷酸终止密码子不信号终止蛋白质合成的报道。为了确定什么构成有效的终止信号，我们分析了来自各种真核生物物种和基因家族的基因中天然终止密码子周围的区域。某些终止密码子和终止密码子之后的核苷酸是过度表达的，并且这种模式在高表达基因中加重。例如，酿酒酵母和黑腹果蝇高度表达基因的优选信号是UAAG，并且通常信号UAA（A/G）和UGA（A/G）在真核生物中是优选的。生物体或DNA区域的GC%可以影响在第二或第四位置中是否存在A或G。因此，我们认为终止密码子和它后面的核苷酸包含四核苷酸终止信号。提出了一种模型，其中的多肽链释放因子，蛋白质，承认这个序列，但会容忍一些取代，特别是在第二或第三个位置的A到G。
An increasing number of cases where tri-nucleotide stop codons do not signal the termination of protein synthesis are being reported. In order to identify what constitutes an efficient stop signal, we analysed the region around natural stop codons in genes from a wide variety of eukaryotic species and gene families. Certain stop codons and nucleotides following stop codons are over-represented, and this pattern is accentuated in highly expressed genes. For example, the preferred signal for Saccharomyces cervisiae and Drosophila melanogaster highly expressed genes is UAAG, and generally the signals UAA(A/G) and UGA(A/G) are preferred in eukaryotes. The GC% of the organism or DNA region can affect whether there is A or G in the second or fourth positions. We suggest therefore, that the stop codon and the nucleotide following it comprise a tetra-nucleotide stop signal. A model is proposed in which the polypeptide chain release factor, a protein, recognises this sequence, but will tolerate some substitution, particularly A to G in the second or third positions.