Cerebrospinal fluid cytokine levels in type 1 narcolepsy patients very close to onset.
Cerebrospinal fluid cytokine levels in type 1 narcolepsy patients very close to onset.
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DOI：
10.1016/j.bbi.2015.03.004
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Mignot E
中科院分区：
文献类型：
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作者：
Kornum BR;Pizza F;Knudsen S;Plazzi G;Jennum P;Mignot E
1 型发作性睡病是由大脑中下丘脑分泌素（食欲素）信号缺失引起的。遗传数据表明，这种疾病是由下丘脑中产生下丘脑分泌素的神经元受到自身免疫攻击引起的。然而，这一假设尚未得到患者样本中自身反应性抗体或 T 细胞的一致发现所证实。对这些阴性结果的一种解释可能是，当患者就诊时，自身免疫过程不再活跃。近年来，随着人们认识的提高，越来越多的患者在疾病发作时被诊断出来。在这项研究中，我们测试了是否可以在这些患者中检测到大脑中的主动免疫过程，如脑脊液（CSF）中细胞因子水平升高所反映的那样。通过多重分析，我们测量了 40 名不同病程的 1 型发作性睡病患者脑脊液中 51 种细胞因子和趋化因子的水平。为了进行比较，我们使用了 9 名健康对照者和 9 名患有其他中枢性嗜睡症患者的样本。对照组和患者之间的细胞因子水平没有显着差异，甚至在脑脊液采样前不到一个月发病的 5 名患者中也是如此。
Type 1 Narcolepsy is caused by a loss of hypocretin (orexin) signaling in the brain. Genetic data suggests the disorder is caused by an autoimmune attack on hypocretin producing neurons in hypothalamus. This hypothesis has however not yet been confirmed by consistent findings of autoreactive antibodies or T-cells in patient samples. One explanation for these negative results may be that the autoimmune process is no longer active when patients present to the clinic. With increasing awareness in recent years, more and more patients have been diagnosed closer and closer to disease onset. In this study, we tested whether an active immune process in the brain could be detected in these patients, as reflected by increased cytokine levels in the cerebrospinal fluid (CSF). Using multiplex analysis, we measured the levels of 51 cytokines and chemokines in the CSF of 40 Type 1 Narcolepsy patients having varying disease duration. For comparison, we used samples from 9 healthy controls and 9 patients with other central hypersomnia. Cytokine levels did not differ significantly between controls and patients, even in 5 patients with disease onset less than a month prior to CSF sampling.