Relationship between Rh-RTH1 and ethylene receptor gene expression in response to ethylene in cut rose
Relationship between Rh-RTH1 and ethylene receptor gene expression in response to ethylene in cut rose
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DOI：
10.1007/s00299-010-0875-z
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发表时间：
2010-06
影响因子：
6.2
通讯作者：
Yixun Yu;Jing Wang;Huina Wang;Zhao-qi Zhang;Juan-xu Liu
中科院分区：
文献类型：
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作者：
Yixun Yu;Jing Wang;Huina Wang;Zhao-qi Zhang;Juan-xu Liu
从衰老玫瑰（Rosa hybridacv）的总RNA中获得了一个推测为rte1样蛋白（Rh-RTH1）的cDNA克隆。使用RT-PCR和RACE技术鉴定花瓣。cDNA全长1061 bp，包含684 bp的开放阅读框，对应227个氨基酸。氨基酸序列与拟南芥RTH、RTE1、番茄GRL2、GRL1和GR的同源性分别为60.0、49.6%、61.2、42.5和39.8%。Northern杂交表明，花瓣和雌蕊中rh - rth1的表达在内源和外源乙烯的作用下增强，在1-MCP的作用下受到抑制。rh - rth1的表达与乙烯受体generh - etr1和rh - etr3的表达位点部分相关，如花瓣、雌蕊、根和芽。1-MCP处理显著抑制了rh - rth1和rh - etr3表达的诱导，而1-MCP处理未降低erh - etr1的表达。用蔗糖处理花后，花瓣和雌蕊中rh - rth1和rh - etr3mrna的水平仅略有下降。损伤处理后，Rh-RTH1、rh - etr1和rh - etr3 mRNA积累量快速增加，与乙烯产量增加呈正相关。rh - rth1与rh - etr1和rh - etr3的表达呈部分相关。
A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybridacv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6, 61.2, 42.5 and 39.8% identity with that of Arabidopsis RTH, RTE1, tomato GRL2, GRL1 and GR, respectively. Northern hybridization indicated thatRh-RTH1expression is enhanced by endogenous and exogenous ethylene and inhibited by 1-MCP in petals and gynoecia.Rh-RTH1expression partly correlated with sites of the ethylene receptor geneRh-ETR1andRh-ETR3expression, such as the petals, gynoecia, roots, and buds. The induction ofRh-RTH1andRh-ETR3expression was substantially suppressed by 1-MCP treatment, whileRh-ETR1expression was not reduced by 1-MCP treatment. Following treatment of flowers with sucrose, the level ofRh-RTH1andRh-ETR3mRNA was only slightly decreased in petals and gynoecia. Upon wounding treatment,Rh-RTH1,Rh-ETR1andRh-ETR3showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The expression ofRh-RTH1showed partial correlation with that ofRh-ETR1andRh-ETR3.