Initiation of inflammation and cell death during liver abscess formation by Entamoeba histolytica depends on activity of the galactose/N-acetyl-D-galactosamine lectin
Initiation of inflammation and cell death during liver abscess formation by Entamoeba histolytica depends on activity of the galactose/N-acetyl-D-galactosamine lectin
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DOI：
10.1016/j.ijpara.2006.10.008
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发表时间：
2007-03-01
影响因子：
4
通讯作者：
Guillen, Nancy
中科院分区：
文献类型：
--
作者：
Blazquez, Samantha;Rigothier, Marie-Christine;Guillen, Nancy
寄生虫溶组织内阿米巴定植于人体肠道，引起阿米巴结肠炎，并通过血管途径传播，形成肝脓肿。Gal/GalNAc凝集素是一种粘附蛋白复合物，可维持大肠杆菌的组织侵袭。溶组织剂在工程寄生虫（HGL-2滋养体）中Gal/GalNAc凝集素功能的破坏改变了仓鼠肝脓肿形成的病理生理学。HGL-2滋养体在血管附近产生许多小的炎症灶。与野生型或对照载体感染相比，HGL-2滋养体向肝组织的低渗透性与中性粒细胞和巨噬细胞对浸润区域的弱吸引力以及促炎性肿瘤坏死因子的缺乏相关。在HGL-2感染中，低宿主炎症反应与肝细胞凋亡延迟相关，而未检测到内皮细胞凋亡。在两种宿主细胞类型中触发细胞凋亡最可能在调节炎症中起核心作用，这是肝阿米巴病的主要标志。这些数据突出了Gal/GalNAc凝集素在启动溶组织大肠杆菌肝脏感染中的关键作用。(c)2006年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
The parasite Entamoeba histolytica colonizes the human intestine causing amoebic colitis and disseminates through the vascular route to form liver abscesses. The Gal/GalNAc lectin is an adhesion protein complex which sustains tissue invasion by E. histolytica. Disruption of the Gal/GalNAc lectin function in engineered parasites (HGL-2 trophozoites) changed the pathophysiology of hamster liver abscess formation. HGL-2 trophozoites produced numerous small inflammatory foci located in the vicinity of blood vessels. The low penetration of HGL-2 trophozoites into hepatic tissue was shown to be associated with weak attraction of neutrophils and macrophages to the infiltrated areas and absence of pro-inflammatory tumour necrosis factor, in contrast to wild type or control vector infections. The low host inflammatory response in HGL-2 infections correlated with a delay in apoptosis of hepatic cells, whereas apoptosis of endothelial cells was not detected. Triggering of apoptosis in both host cell types most likely has a central role in modulating inflammation, a major landmark in hepatic amoebiasis. These data highlight the key role of the Gal/GalNAc lectin in initiation of E histolytica hepatic infection. (c) 2006 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.