Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)
Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)
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DOI：
10.1046/j.1365-3083.2003.01315.x
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发表时间：
2003-09-01
影响因子：
3.7
通讯作者：
O'Brien, RL
中科院分区：
文献类型：
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作者：
Born, WK;Vollmer, M;O'Brien, RL
研究发现表达鼠 γδ T 细胞受体的杂交瘤可响应心磷脂 (CL) 和结构相关的阴离子磷脂而产生细胞因子。这种反应需要血清浓度与培养物中 CL 的量相关。纯化的血清因子 β(2)-糖蛋白 I (β(2)-GP1)（载脂蛋白 H）单独支持 CL 反应，而其他几种血清蛋白和卵清蛋白则不支持。 beta(2)-GP1 已知可与阴离子磷脂（特别是 CL）形成复合物，通常可被病理性自身抗体识别。我们推测 gammadelta T 细胞也识别此类复合物，并且此处报告的杂交瘤反应反映了这种特异性。
Hybridomas expressing murine gammadelta T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, beta(2)-glycoprotein I (beta(2)-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. beta(2)-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that gammadelta T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.