Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins
Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins
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DOI：
10.1128/iai.65.12.5035-5041.1997
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发表时间：
1997-12-01
影响因子：
3.1
通讯作者：
Bush, CA
中科院分区：
文献类型：
--
作者：
Cisar, JO;Sandberg, AL;Bush, CA
凝集素介导的口腔草绿色链球菌和放线菌之间的相互作用可能在牙齿表面的微生物定植中起重要作用。在5种链球菌菌株的细胞壁多糖中存在两种宿主样基序，即GalNAc β 1->3Gal（Gn）或Gal β 1->3GalNAc（G），这解释了这些细菌与内氏放线菌的乳糖敏感性共聚集。具有含Gn多糖的三种链球菌菌株也参与与戈登链球菌和S.每个含Gn或G的多糖由不同的磷酸二酯连接的六糖或七糖重复单元组成。对另外19株与放线菌乳糖敏感性共凝集的草绿色组链球菌进行了检测。用变溶素-细胞壁凝集素阴离子交换柱层析法从17株菌株中分离出了能与羊蹄甲凝集素（一种Gal和GalNAc结合的植物凝集素）反应的带负电荷的多糖。从核磁共振和免疫扩散的结果确定了16个多糖作为一个已知的含Gn或G的结构类型和一个多糖作为一个新的，但密切相关的含Gn的类型。与凝集素的反应不同，大多数兔抗血清与这些多糖的交叉反应与宿主样基序以外的结构特征相关。含Gn多糖主要存在于S. sanguis和S. oralis的多糖含量最高，而含G多糖在S. gordonii和S.缓解检查。这些研究结果强烈支持这一假设，即凝集素介导的识别这些链球菌由其他口腔细菌依赖于一个家庭的抗原多样性的Gn和G-含细胞壁多糖，其发生可能不同的链球菌物种。
Lectin-mediated interactions between oral viridans group streptococci and actinomyces may play an important role in microbial colonization of the tooth surface. The presence of two host-like motifs, either GalNAc beta 1-->3Gal (Gn) or Gal beta 1-->3GalNAc (G), in the cell wall polysaccharides of five streptococcal strains accounts for the lactose-sensitive coaggregations of these bacteria with Actinomyces naeslundii. Three streptococcal strains which have Gn-containing polysaccharides also participate in GalNAc-sensitive coaggregations with strains of Streptococcus gordonii and S. sanguis, Each Gn-or G-containing polysaccharide is composed of a distinct phosphodiester-linked hexa-or heptasaccharide repeating unit. The occurrence of these polysaccharides on 19 additional viridans group streptococcal strains that participate in lactose-sensitive coaggregations with actinomyces was examined, Negatively charged polysaccharides that reacted with Bauhinia purpurea agglutinin, a Gal and GalNAc binding plant lectin, were isolated from 17 strains by anion exchange column chromatography of mutanolysin-cell wall digests. Results from nuclear magnetic resonance and immunodiffusion identified each of 16 polysaccharides as a known Gn-or G-containing structural type and one polysaccharide as a new but closely related Gn-containing type. Unlike the reactions of lectins, the cross-reactions of most rabbit antisera with these polysaccharides were correlated with structural features other than the host-like motifs. Gn-containing polysaccharides occurred primarily on the strains of S. sanguis and S. oralis while G-containing polysaccharides were more common among the strains of S. gordonii and S. mitis examined. The findings strongly support the hypothesis that lectin-mediated recognition of these streptococci by other oral bacteria depends on a family of antigenically diverse Gn-and G-containing cell wall polysaccharides, the occurrence of which may differ between streptococcal species.