Mode of Birth Influences Preterm Infant Intestinal Colonization With Bacteroides Over the Early Neonatal Period.
Mode of Birth Influences Preterm Infant Intestinal Colonization With Bacteroides Over the Early Neonatal Period.
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DOI：
10.1097/anc.0000000000000237
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Gregas M
中科院分区：
文献类型：
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作者：
Gregory KE;LaPlante RD;Shan G;Kumar DV;Gregas M
婴儿期的肠道定植对短期和长期的健康结果都很重要。类杆菌是肠道微生物组的早期成员，是分解肠道内复杂分子所必需的，它的功能是帮助人体免疫系统对抗潜在的有害病原体。新生儿早期类杆菌在早产儿体内的定植模式知之甚少。这项研究基于临床因素，包括出生方式、抗生素和营养，测量了早产儿早期新生儿时期类杆菌的定植情况。从29例早产儿的144份粪便标本中提取细菌DNA，用实时定量聚合酶链式反应(PCR)进行分析。分析包括线性混合模型，以确定哪些临床因素影响婴儿肠道中类杆菌的定植。我们发现，通过阴道出生的婴儿比通过剖腹产出生的婴儿类杆菌的增长率更高(p<；.001)。我们没有发现抗生素使用和营养暴露差异与类杆菌定植之间的显著关联。这些发现突出了出生方式对类杆菌定植的显著影响。虽然出生模式并不总是可以改变的，但这些研究结果可能有助于开发针对通过剖腹产出生的早产儿的干预措施，旨在克服延迟的类杆菌定植。需要对肠道微生物群和与早产儿相关的临床因素进行更多的研究，以便开发和测试干预措施，从而实现最佳的微生物和免疫健康。
Intestinal colonization during infancy is important to short and long term health outcomes. Bacteroides, an early member of the intestinal microbiome, are necessary for breaking down complex molecules within the intestine and function to assist the body’s immune system in fighting against potentially harmful pathogens. Little is known about the colonization pattern of Bacteroides in preterm infants during the early neonatal period. This study measured Bacteroides colonization during the early neonatal period in a population of preterm infants based on clinical factors including mode of birth, antibiotics, and nutrition. Bacterial DNA was isolated from 144 fecal samples from 29 preterm infants and analyzed using quantitative real time polymerase chain reaction (PCR). Analyses included liner mixed models to determine which clinical factors affect Bacteroides colonization of the infant gut. We found that infants born via vaginal canal had a higher rate of increase in Bacteroides than infants born via Cesarean section (p<.001). We did not find significant associations between antibiotic administration and differences in nutritional exposures with Bacteroides colonization. These findings highlight the significant influence of mode of birth on Bacteroides colonization. While mode of birth is not always modifiable, these study findings may help develop interventions for preterm infants born via Cesarean section aimed at overcoming delayed Bacteroides colonization. Greater study of the intestinal microbiome and the clinical factors relevant to the preterm infant is needed so that interventions may be developed and tested, resulting in optimal microbial and immune health.