THE STRUCTURE OF THE STALK SURFACE-LAYER OF A BRINE POND MICROORGANISM - CORRELATION AVERAGING APPLIED TO A DOUBLE LAYERED LATTICE STRUCTURE
THE STRUCTURE OF THE STALK SURFACE-LAYER OF A BRINE POND MICROORGANISM - CORRELATION AVERAGING APPLIED TO A DOUBLE LAYERED LATTICE STRUCTURE
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DOI：
10.1111/j.1365-2818.1985.tb04662.x
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发表时间：
1985-01-01
影响因子：
2
通讯作者：
FRANK, J
中科院分区：
文献类型：
--
作者：
KESSEL, M;RADERMACHER, M;FRANK, J
肢体卤素微生物的茎的表面层是一个周期性阵列（太空群P3M1），该阵列由电子密集的三聚体组成，中心向中心间距为9.0 nm。该结构让人联想到大肠杆菌孔蛋白。相关平均方法可有效地用于分离重叠的晶格，与已建立的傅立叶过滤方法相比，这会导致更高的保真度重建。两种统计方法用于确定相关平均值的分辨率，这是平均窗口数量的函数。傅立叶空间的空间频率的相位残差是在独立获得的亚大规模之间计算的，Q因子分析的新方法研究了傅立叶空间中的累积矢量总和，这是平均窗口数量的函数。两种方法均分辨率为1/2.1 nm-1。
The surface layer of the stalk of a prosthecate halophilic microorganism is a periodic array (space group p3m1) comprised of electron dense trimers with a center to center spacing of 9.0 nm. The structure is reminiscent of Escherichia coli porin. The method of correlation averaging can be effectively used to separate overlapping lattices and this results in a higher fidelity reconstruction, when compared to the established method of Fourier filtration. Two statistical methods are used to determine the resolution of correlation average as a function of the number of windows averaged. The phase residual of spatial frequencies of Fourier space is computed between independently obtained subaverages and a new method of Q factor analysis examines the cumulative vector sum in Fourier space as a function of the number of windows averaged. Both methods give a resolution of 1/2.1 nm-1.