Protective antibody therapy is associated with reduced chemokine transcripts in herpes simplex virus type 1 corneal infection.
Protective antibody therapy is associated with reduced chemokine transcripts in herpes simplex virus type 1 corneal infection.
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保护性抗体治疗与 1 型单纯疱疹病毒角膜感染中趋化因子转录物减少有关。
DOI：
10.1128/jvi.70.2.1277-1281.1996
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Lausch,RN
中科院分区：
文献类型：
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作者：
Su,YH;Yan,XT;Oakes,JE;Lausch,RN
1型单纯疱疹病毒（HSV-1）感染小鼠角膜引起强烈的炎症反应，可导致失明。这种疾病被称为疱疹间质角膜炎，可以通过及时被动转移针对病毒糖蛋白D （gD）的单克隆抗体来预防。确切地说，抗体治疗如何防止角膜过度炎症尚不清楚。在这项研究中，我们研究了趋化因子mRNA的表达是否被抗体治疗抑制。从正常角膜和病毒感染后不同时间分离的总细胞rna通过逆转录- pcr分析编码七种不同趋化因子的mRNA。检测未感染BALB/c小鼠角膜中IP-10、KC、MIP-2、MCP-1、MIP-1 β和RANTES mRNA的组成水平。当角膜受到机械损伤时，所有六种趋化因子的信息都暂时高于构成水平。相比之下，HSV-1感染导致趋化因子信息表达的延长。每种趋化因子的mRNA积累动力学是不同的。直到感染后第7天，MIP-1 α信号才被检测到。感染后1天给予抗hsv gD单克隆抗体与MIP-2、MCP-1、MIP-1 α和MIP-1 β的信息减少相关。IP-10、KC和RANTES消息没有改变。总的来说，我们的结果表明，抗gd治疗可能通过抑制被认为促进炎症的趋化因子的产生来保护，至少在一定程度上。
Herpes simplex virus type 1 (HSV-1) infection on the murine cornea induces an intense inflammatory response which can lead to blindness. This disease, known as herpes stromal keratitis, can be prevented by the timely passive transfer of monoclonal antibody specific for viral glycoprotein D (gD). Precisely how antibody treatment prevents excessive corneal inflammation is not known. In this study we investigated whether chemokine mRNA expression is inhibited by antibody treatment. Total cellular RNAs isolated from normal corneas and at various times after virus infection were analyzed via reverse transcription-PCR for mRNA coding for seven different chemokines. Constitutive levels of IP-10, KC, MIP-2, MCP-1, MIP-1 beta, and RANTES mRNA were detected in uninfected corneas of BALB/c mice. When the cornea was mechanically traumatized, message for all six chemokines was transiently elevated above constitutive levels. In contrast, HSV-1 infection resulted in prolonged enhanced chemokine message expression. The kinetics of mRNA accumulation was distinctive for each chemokine analyzed. MIP-1 alpha message, not detected constitutively, was not evident until day 7 postinfection. Administration of anti-HSV gD monoclonal antibody 1 day after infection was associated with reduced message for MIP-2, MCP-1, MIP-1 alpha, and MIP-1 beta. IP-10, KC, and RANTES messages were not altered. Collectively, our results suggest that anti-gD treatment may protect, at least in part, by inhibiting production of chemokines believed to promote inflammation.