In vivo behavior of calcium phosphate scaffolds with four different pore sizes
In vivo behavior of calcium phosphate scaffolds with four different pore sizes
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DOI：
10.1016/j.biomaterials.2006.05.051
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发表时间：
2006-10-01
期刊：
影响因子：
14
通讯作者：
Auer, Jorg
中科院分区：
文献类型：
--
作者：
von Doernberg, Marie-Cecile;von Rechenberg, Brigitte;Auer, Jorg
本研究的目的是评估大孔尺寸对陶瓷支架体内行为的影响。为此，β-磷酸三钙（β-TCP）圆柱体与四个不同的大孔尺寸（150，260，510，和1220 μ m）被植入钻孔缺损的松质骨的绵羊和他们的吸收行为，随后为6，12和24周。评价支架的生物相容性，并观察新骨形成的宏观，组织学和组织形态计量学。对整个缺损区域和细分为三个同心环（外环、内环和内环）的区域进行组织形态学测量。所有植入物的耐受性均非常好，表现为炎性细胞数量较少且无肉眼可见的炎症体征。由于植入24周时陶瓷残留量不足5%，因此吸收速度较快。几乎没有观察到大孔尺寸对体内反应的任何影响。具有中等大孔尺寸（510 μ m）的样品比具有较小大孔尺寸（150和260 μ m）的样品吸收得更快。然而，这种快速吸收与较低的骨含量和较高的软组织含量相关。在12周和24周时，后者的差异消失了。6周时，外环中的骨比其余块中的骨更丰富，12周时，外环和内环中的骨比内环中的骨更丰富。(c)2006爱思唯尔有限公司保留所有权利。
The goal of the present study was to assess the effect of macropore size on the in vivo behavior of ceramic scaffolds. For that purpose, beta-tricalcium phosphate (beta-TCP) cylinders with four different macropore sizes (150, 260, 510, and 1220 mu m) were implanted into drill hole defects in cancellous bone of sheep and their resorption behavior was followed for 6, 12 and 24 weeks. The scaffolds were evaluated for biocompatibility, and new bone formation was observed macroscopically, histologically and histomorphometrically. Histomorphometrical measurements were performed for the whole defect area and for the area subdivided into three concentric rings (outer, medial, and inner ring). All implants were tolerated very well as evidenced by the low amount of inflammatory cells and the absence of macroscopic signs of inflammation. Resorption proceeded fast since less than 5% ceramic remained at 24-week implantation. Hardly any effect of macropore size was observed on the in vivo response. Samples with an intermediate macropore size (510 mu m) were resorbed significantly faster than samples with smaller macropore sizes (150 and 260 pm). However, this fast resorption was associated with a lower bone content and a higher soft tissue content. At 12 and 24 weeks, the latter differences had disappeared. Bone was more abundant in the outer ring than in the rest of the blocks at 6 weeks, and in the outer and medial ring compared to the inner ring at 12 weeks. (c) 2006 Elsevier Ltd. All rights reserved.