Reversible and irreversible effects of chemical fixation on the NMR properties of single cells
Reversible and irreversible effects of chemical fixation on the NMR properties of single cells
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DOI：
10.1002/mrm.21018
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发表时间：
2006-10-01
影响因子：
3.3
通讯作者：
Webb, Andrew G.
中科院分区：
文献类型：
--
作者：
Purea, Armin;Webb, Andrew G.
众所周知，化学固定的影响会改变磁共振参数，如松弛时间和水的表观扩散系数(ADC)。通常认为，在将样品重新浸入缓冲液中足够长的时间后，这种变化是可逆的。在这项研究中，我们描述了非洲爪哇单个卵母细胞的固定和随后在缓冲液中重新浸泡的变化。固定后细胞各隔室T-1和T-2值均明显降低，胞浆的变化大于胞核。在缓冲液中重新浸泡后，新鲜细胞和重新浸泡细胞之间的MR参数差异很小，但有统计学意义。使用Gd造影剂的实验表明，细胞膜对小分子的通透性发生了不可逆转的变化。
The effects of chemical fixation are known to alter MR parameters, such as relaxation times and the apparent diffusion coefficient (ADC) of water. It is often assumed that such change are reversible after samples have been reimmersed in a buffer solution for a sufficient period of time. In this study we characterize the changes associated with fixation of single Xenopus laevis oocytes and their subsequent reimmersion in buffer. Sub stantial reductions in both T-1 and T-2 values were measured for all compartments of the cell after fixation, with the cytoplasm showing larger changes than the nucleus. After reimmersion in buffer, there were small but statistically significant difference in MR parameters between fresh and reimmersed cells. Experiments with a gadolinium (Gd) contrast agent showed evidence of irreversible changes in the permeability of cellular membrane., to small molecules.