EXCITATORY AND INHIBITORY AUTAPTIC CURRENTS IN ISOLATED HIPPOCAMPAL-NEURONS MAINTAINED IN CELL-CULTURE
EXCITATORY AND INHIBITORY AUTAPTIC CURRENTS IN ISOLATED HIPPOCAMPAL-NEURONS MAINTAINED IN CELL-CULTURE
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DOI：
10.1073/pnas.88.17.7834
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
STEVENS, CF
中科院分区：
文献类型：
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作者：
BEKKERS, JM;STEVENS, CF
个别大鼠海马神经元，生长在隔离从其他神经元上的小斑点的许可基板，进行了研究，以表征的电特性的突触，这些细胞形成与自己（autapses）。兴奋性（可能是谷氨酸能）或抑制性（可能是A型γ-氨基丁酸能）autapses. Excitatory autaptic电流逆转附近的电位预期的单价阳离子被阻断的谷氨酸能拮抗剂犬尿烯酸，并拥有一个缓慢的组成部分，药理学的N-甲基-D-天冬氨酸型通道。这些电流也表现出试验到试验的统计波动，其振幅，这是很好地描述了量子分析。抑制性自适应电流逆转超极化电位，预期氯渗透孔，并被阻断印防己毒素，一种A型γ-氨基丁酸受体拮抗剂。它的结论是，autaptic电流在文化中是相同的那些发现在突触。
Individual rat hippocampal neurons, grown in isolation from other neurons on small spots of permissive substrate, were studied in order to characterize the electrical properties of the synapses that such cells formed with themselves (autapses). Excitatory (probably glutamatergic) or inhibitory (probably type A gamma-aminobutyratergic) autapses were frequently found. Excitatory autaptic currents reversed near the potential expected for monovalent cations were blocked by the glutamatergic antagonist kynurenic acid, and possessed a slow component with the pharmacological profile of N-methyl-D-aspartate-type channels. These currents also exhibited trial-to-trial statistical fluctuations in their amplitudes, this being well-described by quantal analysis. Inhibitory autaptic currents reversed at hyperpolarized potentials, as expected for chloride-permeable pores and were blocked by picrotoxin, a type A gamma-aminobutyric receptor antagonist. It is concluded that autaptic currents in culture are identical to those found at synapses.