Optimized assay for mammalian tyrosinase (polyhydroxyl phenyloxidase).
Optimized assay for mammalian tyrosinase (polyhydroxyl phenyloxidase).
复制标题
哺乳动物酪氨酸酶（多羟基苯基氧化酶）的优化测定。
DOI：
10.1016/0003-2697(84)90015-0
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
King,RA
中科院分区：
文献类型：
--
作者：
Townsend,D;Guillery,P;King,RA
酪氨酸酶是动物界色素形成中的重要酶，已经描述了许多测定其活性的方法。本文介绍了一种测定方法，它已被优化，以测量哺乳动物酪氨酸酶的酶活性，并描述了程序，从其中确定的最佳条件。还详细描述了进行测定所需储备试剂的制备和储存，以及与测定前样品制备有关的一些讨论。
Tyrosinase is an important enzyme in the formation of pigment throughout the animal kingdom and many assays have been described for measuring its activity. This paper describes an assay which has been optimized to measure the enzymatic activity of mammalian tyrosinase and describes the procedures from which the optimum conditions were determined. The preparation and storage of the stock reagents required to perform the assay are also described in detail as well as some discussion pertaining to the preparation of samples prior to assay.