Influence of subterminal viral DNA nucleotides on differential susceptibility to cleavage by human immunodeficiency virus type 1 and visna virus integrases.
Influence of subterminal viral DNA nucleotides on differential susceptibility to cleavage by human immunodeficiency virus type 1 and visna virus integrases.
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亚末端病毒 DNA 核苷酸对人类免疫缺陷病毒 1 型和维斯纳病毒整合酶裂解的不同敏感性的影响。
DOI：
10.1128/jvi.70.12.9069-9073.1996
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Sudol,M
中科院分区：
文献类型：
--
作者：
Katzman,M;Sudol,M
比较U5和U3病毒DNA末端的位点特异性切割的程度的整合酶的人类免疫缺陷病毒1型和visna病毒指导的定量检测含有特定的碱基取代的寡核苷酸底物。U3基板之间的位置5和6的同时交换切换模式的差异敏感性的两个整合酶。维斯纳病毒整合酶的活性更依赖于位置5的身份比位置6上的不变的CA碱基相邻，而人类免疫缺陷病毒1型整合酶似乎与位置6更关键的相互作用。尽管大多数慢病毒整合酶的配对天然底物在位置7和8处匹配，但这些碱基对于U5底物的易感性并不重要。事实上，最后六个U5位置包含易感性所需的所有序列信息。这些结果表明，整合以外的限制影响逆转录病毒DNA的末端反向重复序列。
A comparison of the extents of site-specific cleavage of U5 and U3 viral DNA termini by the integrases of human immunodeficiency virus type 1 and visna virus guided the quantitative testing of oligonucleotide substrates containing specific base substitutions. The simultaneous exchange of positions 5 and 6 between U3 substrates switched the patterns of differential susceptibility to the two integrases. The activity of visna virus integrase was more dependent on the identity of position 5 adjacent to the invariant CA bases than on position 6, whereas human immunodeficiency virus type 1 integrase appeared to interact even more critically with position 6. Although the paired natural substrates of most lentiviral integrases match at positions 7 and 8, these bases were not important for susceptibility of U5 substrates. In fact, the final six U5 positions contained all of the sequence information necessary for susceptibility. These results suggest that constraints other than integration influence the terminal inverted repeats of retroviral DNA.