Complete genome sequence of a novel fusarivirus from Rhizoctonia solani AG-3 PT strain 3P-2-2
Complete genome sequence of a novel fusarivirus from Rhizoctonia solani AG-3 PT strain 3P-2-2
复制标题
立枯丝核菌 AG-3 PT 株 3P-2-2 新型镰孢病毒的完整基因组序列
DOI：
10.1007/s00705-022-05673-7
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发表时间：
2023
影响因子：
2.7
通讯作者：
Genhua Yang
中科院分区：
文献类型：
--
作者：
Aili Sun;Li;Z. Zhao;Lijuan Kang;Chengyun Li;W. Dong;Genhua Yang
在这里，我们描述了一种新的真菌病毒，暂命名为“立枯丝核菌镰刀菌病毒6”（RsFV 6），这是发现于立枯丝核菌AG-3 PT株3 P-2-2。该病毒具有6141个核苷酸的单链正义RNA（+ssRNA）基因组，含有两个开放阅读框（ORF）和一个poly（A）尾。ORF 1编码一个1，862个氨基酸的大多肽，具有保守的RNA依赖性RNA聚合酶（RdRp）和解旋酶（Hel）结构域。ORF 2编码一个由167个氨基酸组成的功能未知的蛋白质。BLASTp搜索结果显示，ORF 1编码的多肽与分离自立枯丝核菌AG-2-2LP的立枯丝核菌镰刀菌病毒3（RsFV 3）的序列相似性最高（70.67%）。基于RdRp和Hel序列的多重序列比对和系统发育分析表明，RsFV 6可能是镰刀病毒科α镰刀病毒属的一个新成员。
Here, we describe a novel mycovirus, tentatively designated as “Rhizoctonia solani fusarivirus 6” (RsFV6), which was discovered in Rhizoctonia solani AG-3 PT strain 3P-2-2. The virus has a single-stranded positive-sense RNA (+ssRNA) genome of 6141 nucleotides containing two open reading frames (ORFs) and a poly(A) tail. ORF1 encodes a large polypeptide of 1,862 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF2 encodes a putative 167-aa protein of unknown function. BLASTp searches revealed that the ORF1-encoded polypeptide showed the highest sequence similarity (70.67% identity) to that of Rhizoctonia solani fusarivirus 3 (RsFV3), which was isolated from Rhizoctonia solani AG-2-2LP. Multiple sequence alignments and phylogenetic analysis based on RdRp and Hel sequences indicated that RsFV6 could be a novel member of the genus Alphafusarivirus family Fusariviridae.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
2.5
作者：
Gonzalez, Dolores;Rodriguez-Carres, Marianela;Boekhout, Teun;Stalpers, Joost;Kuramae, Eiko E.;Nakatani, Andreia K.;Vilgalys, Rytas;Cubeta, Marc A.
通讯作者：
Cubeta, Marc A.