Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes
Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes
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DOI：
10.1111/2041-210x.12834
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发表时间：
2017-12
影响因子：
6.6
通讯作者：
Jun Matsubayashi;Y. Saitoh;Yutaka Osada;Yoshitoshi Uehara;J. Habu;Tsuyoshi Sasaki;I. Tayasu
中科院分区：
文献类型：
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作者：
Jun Matsubayashi;Y. Saitoh;Yutaka Osada;Yoshitoshi Uehara;J. Habu;Tsuyoshi Sasaki;I. Tayasu
同位素分析在了解水生生态系统中鱼类生态和食物网结构方面具有很高的潜力。如果我们能够在单个鱼的多个生长阶段重建几种同位素的年代学，将大大提高同位素分析的实用性。然而，目前还没有实用的方法来重建硬骨鱼的轻元素同位素（如δ13C、δ15N、δ34S和Δ14C）的年代学。在这里，我们提出并测试了一种新的分析方法，用于重建硬骨鱼多个生命阶段的轻同位素同位素比率。我们采集了一种溯河鲑鱼，masu salmon Oncorhynchus masou (n = 3)，以及来自其出生溪流和海洋的水。我们将鲑鱼的椎体中心平均分成10个部分，并从每个样本中提取骨胶原。然后，我们测量了每个椎体截面的稳定硫同位素比率，并将其与河水和海水的δ34S值进行了比较。我们还测量了耳石的87Sr/86Sr比率，作为鲑鱼在淡水和海洋之间迁移的参考指标。在所有样品中，最靠近椎体中心的骨段δ34S值最低，与淡水相似。δ34S值从中心段到边缘段逐渐增大，最终达到与海水相似的恒定值。矢状面耳石剖面的87Sr/86Sr比值具有显著的个体间差异，且与椎体剖面δ34S值的变化规律一致。研究结果表明，硬骨鱼的脊椎中心记录了从幼年到成年的同位素信息。我们认为我们的方法可以提供可重复的同位素年代学，甚至在小于50厘米的硬骨鱼中也是如此。该方法可用于景观研究和海洋硬骨鱼生态学研究。
Isotope analysis has high potential for understanding fish ecology and food‐web structure in aquatic ecosystems. The utility of isotope analysis will be greatly improved if we can reconstruct the chronology of several isotopes at multiple growth stages of individual fish. However, no practical methods exist for reconstructing the chronology of light‐element isotopes (e.g. δ13C, δ15N, δ34S, and Δ14C) in teleost fishes. Here, we present and test a new analytical approach for reconstructing the isotopic ratios of light isotopes at multiple life‐stages in teleost fishes. We sampled an anadromous salmon species, masu salmon Oncorhynchus masou (n = 3), along with water from its natal stream and from the ocean. We subdivided the vertebral centra of the salmon equally into 10 sections and extracted bone collagen from each sample. We then measured the stable sulphur isotope ratios of each vertebral section and compared them with δ34S values of the river water and sea water. We also measured the 87Sr/86Sr ratios of otoliths as a reference indicator of salmon migration between fresh water and the ocean. In all samples, the bone section closest to the centre of the centrum had the lowest δ34S values, which were similar to those of fresh water. The δ34S values gradually increased from the centre to marginal sections, finally reaching constant values similar to those of seawater. The 87Sr/86Sr ratios of sagittal otolith sections had significant inter‐individual differences and were consistent with the patterns of variation of the δ34S values of the vertebral sections. Our results show that the vertebral centra of teleost fishes record isotopic information from juvenile to adult life stages. We suggest that our method can provide reproducible isotopic chronology, even in teleost fishes smaller than 50 cm. This method can be used in isoscape studies and in studies of the ecology of marine teleost fishes.