Systemic concentrations of endogenous and exogenous FSH in anoestrous ewes superstimulated with folltropin-V.
Systemic concentrations of endogenous and exogenous FSH in anoestrous ewes superstimulated with folltropin-V.
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DOI：
10.1111/j.1439-0531.2008.01124.x
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
P. Bartlewski;KJ Fuerst;BD Alexander;WA King
中科院分区：
文献类型：
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作者：
P. Bartlewski;KJ Fuerst;BD Alexander;WA King
在非动情母羊卵巢超刺激之前，用醋酸甲羟孕酮（MAP）和雌二醇-17 β（E（2）-17 β）使卵泡波同步，通过一种未知的机制减少了超排卵反应的变异性。卵泡刺激素（FSH）是一个主要的促进剂窦卵泡发育，但相关性的循环FSH浓度的超数排卵性能的母羊还没有被检查。用促卵泡素-V（猪FSH）对18只非发情的Rideau Arcott母羊（5月至6月）进行超数排卵，其中（n = 8;处理母羊）或不（n = 10;对照母羊）单次i. m. 350 μ g E（2）-17 β，在14天治疗的第6天给予释放MAP的阴道内海绵（60 mg）。注射E（2）-17 β后6天开始超排卵治疗，包括6次i.m.施用Folltropin-V，每天两次（在08：00和16：00 h），然后i.m.注射GnRH（50微克）。在整个3天处理期间，每8小时采集一次血样，使用种属特异性标准品和一抗，通过放射免疫测定法分析绵羊和猪FSH的浓度。在pFSH血清浓度方面，两组之间的oFSH血清浓度更高（p 0.05）。在第一次注射促卵泡素-V后8 h（r = 0.72，p < 0.05）、16 h（r=0.63，p < 0.05）和64 h（r = 0.84，p < 0.01），黄体（CL）数量与oFSH浓度之间记录到显著相关性。回收胚胎数与56 h oFSH浓度呈正相关（r = 0.69，p < 0.05）。我们的结论是，内源性FSH浓度的变化，在卵巢超刺激与pFSH可能有助于母羊超排卵反应的变异。
The synchronization of follicular waves with medroxyprogesterone acetate (MAP) and oestradiol-17beta (E(2)-17beta) prior to ovarian superstimulation in anoestrous ewes reduces the variability in superovulatory responses by an unknown mechanism. Follicle stimulating hormone (FSH) is a primary promoter of antral follicular development, but the relevance of circulating FSH concentrations to the superovulation performance in ewes has not been examined. Eighteen anoestrous Rideau Arcott ewes (May-June) were superovulated with Folltropin-V (porcine FSH), with (n = 8; treated ewes) or without (n = 10; control ewes) a single i.m. dose of 350 microg of E(2)-17beta, given on the sixth day of a 14-day treatment with MAP-releasing intravaginal sponges (60 mg). The superovulatory treatment, begun 6 days after E(2)-17beta injection, consisted of six i.m. applications of Folltropin-V given twice daily (at 08:00 and 16:00 h), followed by an i.m. injection of GnRH (50 microg). Blood samples collected every 8 h throughout the 3-day treatment, were analysed by radioimmunoassays for concentrations of ovine and porcine FSH, using species-specific standards and primary antibodies. Serum concentrations of oFSH were greater (p 0.05) between the two groups in serum concentrations of pFSH. Significant correlations were recorded between the number of corpora lutea (CL) and oFSH concentrations at 8 h (r = 0.72, p < 0.05), 16 h (r=0.63, p < 0.05) and 64 h (r = 0.84, p < 0.01) after the 1(st) Folltropin-V injection. The total number of recovered embryos was positively correlated to oFSH concentrations at 56 h (r = 0.69, p < 0.05). We concluded that changes in endogenous FSH concentrations during ovarian superstimulation with pFSH might contribute to the variability in superovulatory responses in ewes.