[The study of the tumorigenicity and metastasis ability in human lung cancer cell line L9981 using in vivo imaging.].
[The study of the tumorigenicity and metastasis ability in human lung cancer cell line L9981 using in vivo imaging.].
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DOI：
10.3779/j.issn.1009-3419.2008.03.012
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发表时间：
2008-06-20
期刊：
影响因子：
--
通讯作者：
Zhou, Qinghua
中科院分区：
文献类型：
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作者：
Ren, Yuanrong;Chen, Jun;Zhou, Qinghua
背景：通过体内显像技术研究人大细胞肺癌L9981细胞的致瘤性和转移能力。方法：首先将含有萤火虫荧光素酶（luc）基因的质粒转染到L9981细胞中，然后用G418建立稳定转染的L9981-luc细胞系。然后将阳性L9981-Lue细胞皮下植入小鼠体内，利用体内显像技术监测肿瘤生长和微转移情况。结果：结果表明，稳定转染的L9981-Lue细胞的生物发光密度与体外肿瘤细胞数量相关。L9981-Luc细胞仍保持高转移特性。将L9981-Luc细胞植入小鼠皮下数周后，利用体内显像机发现小鼠不同脏器的转移灶，肿瘤的生物发光与肿瘤大小相关。此外，我们通过小鼠疤痕和病理染色分析证实转移灶。结论：我们建立了稳定的高转移性L9981-Luc细胞系，可通过体内显像分析动物模型中肿瘤的侵袭和转移情况。
BACKGROUND: The aim of this work is to study the tumorigenicity and metastasis ability in human large cell lung caner cell line L9981 by in vivo imaging.METHODS: We firstly transfected the plasmids with firefly luciferase (luc ) gene into L9981 cells and then established the stable transfected L9981-luc cell line with G418. Then the positive L9981-Lue cells were implanted subcutaneously into mice and were monitored for tumor growth and micrometastases with in vivo imaging technique.RESULTS: The results showed that the bioluminescence density of the stable transfected L9981-Lue cells correlated to the numbers of the tumor cells in vitro . The L9981-Luc cells still keep the high metastasis characterization. After the L9981-Luc cells were implanted into mice subcutaneously for several weeks, we found the metastasis lesions in the different organs of the mice using in vivo imaging machine and the bioluminescence of the tumor correlated with its size. Furthermore, we confirmed the metastasis lesions by scarifying the mice and analyzing with pathological staining.CONCLUSIONS: We established a stable L9981-Luc cell line with high metastasis character that can be used to analyze the tumor invasion ad metastasis in animal model by in vivo imaging.