Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelectrolyte multilayer films: Correlations between substrate stiffness and cell adhesion
Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelectrolyte multilayer films: Correlations between substrate stiffness and cell adhesion
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DOI：
10.1016/j.susc.2004.06.179
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发表时间：
2004-10-10
期刊：
影响因子：
1.9
通讯作者：
Discher, DE
中科院分区：
文献类型：
--
作者：
Engler, AJ;Richert, L;Discher, DE
薄膜基质以及生物样品的弹性的表面探针测量被证明对于理解跨此类系统的细胞附着通常是重要的。为了说明这一点，通过原子力显微镜（AFM）在富含平滑肌细胞（SMC）的中间层内探测切片动脉，产生类似于5-8 kPa的表观杨氏模量E-介质。聚丙烯酰胺凝胶与E-凝胶跨越几倍以上和以下这个范围，然后铸造5-70 μ m厚，并涂有胶原蛋白：SMC扩展显示出双曲线依赖于投影细胞面积与E-凝胶。给出半最大扩展的模量是E1/2-扩展，类似于8-10 kPa，证明非常接近E1（介质）。还测试了聚（（L）-赖氨酸）/透明质酸的更复杂的逐层微膜，并显示出随着刚度增加而增加的SMC铺展的等同趋势。因此，细胞的粘附扩散似乎与合成材料和组织的有效刚度广泛相关。(C)2004 Elsevier B. V.保留所有权利。
Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by atomic force microscopy (AFM) within the smooth muscle cell (SMC)-rich medial layer, yielding an apparent Young's modulus E-media similar to 5-8 kPa. Polyacrylamide gels with E-gel spanning several-fold above and below this range were then cast 5-70 [mum thick and coated with collagen: SMC spreading shows a hyperbolic dependence in projected cell area versus E-gel. The modulus that gives half-max spreading is E1/2-spread similar to 8-10 kPa, proving remarkably close to E,(media). More complex, layer-by-layer microfilms of poly((L)-lysine)/hyaluronic acid were also tested and show equivalent trends of increased SMC spreading with increased stiffness. Adhesive spreading of cells thus seems to correlate broadly with the effective stiffness of synthetic materials and tissues. (C) 2004 Elsevier B.V. All rights reserved.