Effect of prior treatment with resveratrol on density and structure of rat long bones under tail-suspension
Effect of prior treatment with resveratrol on density and structure of rat long bones under tail-suspension
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DOI：
10.1007/s00774-010-0187-y
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Brasse, David
中科院分区：
文献类型：
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作者：
Habold, Caroline;Momken, Iman;Brasse, David
在太空飞行或长时间卧床休息期间缺乏身体活动会导致人体骨量迅速而显著的损失。白藜芦醇是一种红葡萄酒多酚，目前正在研究其治疗性抗氧化剂特性，已被证明可显著调节骨代谢的生物标志物，即，以促进成骨细胞分化并防止由雌激素缺乏引起的骨丢失。然而，没有直接证据支持其对身体活动期间骨丢失的抑制作用。在本研究中，白藜芦醇对骨密度（BMD），骨矿物质含量和骨结构的影响进行了研究，在股骨和胫骨的尾部悬吊和非悬吊大鼠使用X射线显微计算机断层扫描（micro-CT）。大鼠用400 mg/kg/天的白藜芦醇治疗45天，其中一半在治疗的最后2周暂停。暂停导致胫骨和股骨骨密度下降以及骨小梁和皮质骨退化。悬浮期间的骨退化是由骨髓面积增加引起的，这可能是由具有破骨细胞生成潜能的基质细胞或脂肪细胞增加引起的。白藜芦醇对后肢制动引起的骨丢失有预防作用。特别是，胫骨近端干骺端的骨小梁在尾悬吊前用白藜芦醇处理的大鼠中完全保留。
Physical inactivity during space flight or prolonged bed rest causes rapid and marked loss of bone mass in humans. Resveratrol, a red wine polyphenol that is currently under study for its therapeutic antioxidant properties, has been shown to significantly modulate biomarkers of bone metabolism, i.e., to promote osteoblast differentiation and to prevent bone loss induced by estrogen deficiency. However, there is no direct evidence supporting its inhibitory effect toward bone loss during physical inactivity. In the present study, effects of resveratrol on bone mineral density (BMD), bone mineral content, and bone structure were examined in the femora and tibiae of tail-suspended and unsuspended rats using X-ray micro-computed tomography (micro-CT). Rats were treated with 400 mg/kg/day of resveratrol for 45 days and half of them were suspended during the last 2 weeks of treatment. Suspension caused a decrease in tibial and femoral BMD and deterioration of trabecular and cortical bone. Bone deterioration during suspension was paralleled by increased bone marrow area, which could be caused by an increase in stromal cells with osteoclastogenic potential or in adipocytes. Resveratrol had a preventive effect against bone loss induced by hindlimb immobilization. In particular, trabecular bone in the proximal tibial metaphysis was totally preserved in rats treated with resveratrol before tail-suspension.