IL-8 and TNF-α from peripheral neutrophils and acute-phase proteins in periodontitis -: Effect of cigarette smoking:: a pilot study
IL-8 and TNF-α from peripheral neutrophils and acute-phase proteins in periodontitis -: Effect of cigarette smoking:: a pilot study
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DOI：
10.1034/j.1600-051x.2002.290206.x
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发表时间：
2002-02-01
影响因子：
6.7
通讯作者：
Åsman, B
中科院分区：
文献类型：
--
作者：
Fredriksson, M;Bergström, K;Åsman, B
目的：在其他研究中，我们发现牙周炎患者外周血中性粒细胞功能异常。本研究的目的是研究（1）15例经治疗的牙周炎患者和配对对照组的中性粒细胞释放的细胞因子IL-8和TNF α以及（2）吸烟的影响。材料和方法：用ELISA法测定未受刺激和Fc γ-R刺激的中性粒细胞在孵育培养基中释放的细胞因子和一些急性期反应物。与不吸烟的对照组相比，不吸烟的患者具有较低TNF α释放的趋势，而IL-8的相应趋势相当相似。吸烟对这两种细胞因子的释放有中度但不一致的影响。然而，在患者中，吸烟显著降低了IL-8刺激/非刺激释放的比例（p
Objective: In other studies, we have found deviant functions in peripheral neutrophils in periodontitis. The aim here was to study (1) the release of cytokines, IL-8 and TNFalpha, from neutrophils in 15 treated periodontitis patients and pair-matched controls as well as (2) the effects of cigarette smoking.Material and methods: Cytokines released in the incubation medium from unstimulated and Fcgamma-R-stimulated neutrophils and some acute-phase reactants were measured with ELISA.Results: Non-smoking patients had trends for lower TNFalpha release compared to non-smoking controls, while corresponding trends were rather similar for Il-8. Smoking had a moderate but inconsistent effect on the release of both cytokines. However, in patients, the ratio between stimulated/un-stimulated release of Il-8 was significantly lowered by smoking (p