Linkage map of the honey bee, Apis mellifera, based on RAPD markers.
Linkage map of the honey bee, Apis mellifera, based on RAPD markers.
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发表时间：
1995-03
期刊：
影响因子：
3.3
通讯作者：
Greg J. Hunt;Robert E. Page
中科院分区：
文献类型：
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作者：
Greg J. Hunt;Robert E. Page
利用365个随机扩增多态性DNA（RAPD）标记在单雌蜂的单倍体雄性后代中的分离，构建了一张蜜蜂遗传连锁图。X基因座的性别决定和基因的黑体颜色和苹果酸脱氢酶被映射到单独的连锁群。RAPD标记是非常有效的映射，与平均约2.8个座位映射每个10个核苷酸的引物，用于聚合酶链反应。图上标记之间的平均间隔大小为9.1 cM。该图谱覆盖了26个连锁群上的3110 cM连锁标记。我们估计总基因组大小约为3450 cM。图谱的大小表明蜜蜂的重组率非常高。物理遗传距离的关系估计为52 kb/cM，这表明基于图位的基因克隆对该物种是可行的。
A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be approximately 3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.