The Endophytic Strain Fusarium oxysporum Fo47: A Good Candidate for Priming the Defense Responses in Tomato Roots
The Endophytic Strain Fusarium oxysporum Fo47: A Good Candidate for Priming the Defense Responses in Tomato Roots
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DOI：
10.1094/mpmi-12-12-0290-r
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发表时间：
2013-08-01
影响因子：
3.5
通讯作者：
Olivain, Chantal
中科院分区：
文献类型：
--
作者：
Aime, Sebastien;Alabouvette, Claude;Olivain, Chantal
尖孢镰刀菌Fo 47对番茄枯萎病有较好的防治效果。以前的研究已经证明了直接拮抗作用和诱导抗性的参与。本研究的目的是调查是否引发植物防御反应是一种机制，Fo 47控制枯萎病。一个在体外的设计，使主根接种Fo 47和致病菌株（FoI 8）在不同的位置和不同的时间。已知参与番茄防御反应的六个基因的表达水平使用逆转录定量聚合酶链反应（qPCR）进行定量。三个基因-CHI 3，GLUA，PR-1a-过表达的根预接种Fo 47，然后挑战FoI 8。在接种Fo 47后，GLUA和PR-la基因在子叶中上调。通过qPCR，使用Fo 47和FoI 8的特异性标记物评估根中的真菌生长。结果表明，减少了病原体的生长在番茄植物的根预接种Fo 47。本研究表明，引发番茄防御反应是Fo 47的作用机制之一，其诱导病原体减少根部的定殖。
The protective Fusarium oxysporum strain Fo47 is effective in controlling Fusarium wilt in tomato. Previous studies have demonstrated the role of direct antagonism and involvement of induced resistance. The aim of the present study was to investigate whether priming of plant defense responses is a mechanism by which Fo47 controls Fusarium wilt. An in vitro design enabled inoculation of the tap root with Fo47 and the pathogenic strain (FoI8) at different locations and different times. The expression levels of six genes known to be involved in tomato defense responses were quantified using reverse-transcription quantitative polymerase chain reaction (qPCR). Three genes-CHI3, GLUA, and PR-1a-were overexpressed in the root preinoculated with Fo47, and then challenged with FoI8. The genes GLUA and PR-la were upregulated in cotyledons after inoculation of Fo47. Fungal growth in the root was assessed by qPCR, using specific markers for Fo47 and FoI8. Results showed a reduction of the pathogen growth in the root of the tomato plant preinoculated with Fo47. This study demonstrated that priming of tomato defense responses is one of the mechanisms of action of Fo47, which induces a reduced colonization of the root by the pathogen.