Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed pluripotent stem cell-based differentiation model.
Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed pluripotent stem cell-based differentiation model.
复制标题
在人引发的多能干细胞分化模型中，滋养层样合胞体与羊膜外胚层类似物分层。
DOI：
10.1016/j.celrep.2022.110973
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发表时间：
2022
期刊：
影响因子：
8.8
通讯作者：
Mototsugu Eiraku.
中科院分区：
文献类型：
--
作者：
Masatoshi Ohgushi. Nobuko Taniyama;Alexis Vandenbon;Mototsugu Eiraku.
众所周知，人胚胎干细胞（ESCs）可以转化为具有多种滋养细胞特性的细胞，但这种现象是否代表人胚胎干细胞向滋养细胞谱系的固有分化能力仍然存在争议。在这项研究中，我们报告了激活素/NODAL和FGF信号的化学阻断足以引导人类引物ESCs转化为表达gata3的细胞，从而产生胎盘激素产生的合胞体，类似于人类胚胎着床后阶段的合胞滋养细胞。尽管它们的细胞学与合胞滋养层细胞相似，但这些合胞体起源于非滋养层细胞的分化轨迹，再现了羊膜发生。这些结果提供了对灵长类胚胎发生中可能的独特胚胎外分化途径的见解。
Human primed embryonic stem cells (ESCs) are known to be converted to cells with several trophoblast properties, but it has remained controversial whether this phenomenon represents the inherent differentiation competence of human primed ESCs to trophoblast lineages. In this study, we report that chemical blockage of ACTIVIN/NODAL and FGF signals is sufficient to steer human primed ESCs into GATA3-expressing cells that give rise to placental hormone-producing syncytia analogous to syncytiotrophoblasts of the post-implantation stage of the human embryo. Despite their cytological similarity to syncytiotrophoblasts, these syncytia arise from the non-trophoblastic differentiation trajectory that recapitulates amniogenesis. These results provide insights into the possible extraembryonic differentiation pathway that is unique in primate embryogenesis.