Effects of ethanol on active transport in the dog stomach.
Effects of ethanol on active transport in the dog stomach.
复制标题
乙醇对狗胃主动运输的影响。
DOI：
10.1152/ajplegacy.1974.226.2.397
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
L. L. Shanbour
中科院分区：
文献类型：
--
作者：
T. J. Sernka;C. Gilleland;L. L. Shanbour
方法杂种犬，雌雄不限，体重10 ~ 30 kg，禁食24 h后，用氯醛糖-氨基甲酸乙酯（1：10）605 mg/kg麻醉。股静脉插管用于静脉输注麻醉剂或促分泌剂。进行中线剖腹手术，暴露内脏。脾动脉的胃分支逆行插管进行动脉内输注。切除胃窦以减少胃泌素对胃酸分泌的影响。胃底大弯的楔形物相对于脾动脉分支的胃网膜血管对称地夹紧（插图，图1）。将胃底节段切除，取出，并以一定角度固定在Lucite腔室中，以便通过重力引流，血液供应保持完整（11）。将暴露的胃粘膜用150 mM NaCl（盐水）浸泡1小时或更长时间以达到稳态。完整的腔室组件如图1所示。粘膜和血清电压电极通过连接到甘汞电极的盐水柱和琼脂盐水桥（Radiometer K40 1）检测跨胃粘膜的自发或钳位PD。电流从位于粘膜室溶液中的Ag-AgCl粘膜电极通过粘膜的相同部分到达位于血清盐水柱中的Ag-AgCl血清电极。连续记录PD，除了在自动零位平衡伺服电位计（Electronik 112，Honeywell）上周期性测量电流之外，该电位计被修改以允许电位检测和电流通过的分离，并允许原始电位的20倍放大。
METHODSMongrel dogs of either sex weighing lo-30 kg were anesthetized with cr-chloralose-ethyl carbamate (1: lo), 605 mg/kg, following a 24-h fast. The femoral vein was cannulated for intravenous infusion of anesthetic or secretagogue. A midline laparotomy was performed and the viscera were exposed. A gastric branch of the splenic artery was cannulated retrograde for intra-arterial infusion. The antrum was removed to reduce gastrin efl’ects on acid secretion. A wedge from the greater curvature of the fundus was clamped symmetrically with respect to the gastroepiploic vasculature that branches from the splenic artery (insert, Fig. 1). The fundic segment was cut free, exteriorized, and mounted in a Lucite chamber at an angle for drainage by gravity with the blood supply left intact (11). The exposed gastric mucosa was bathed with 150 mM NaCl (saline) for 1 h or more to reach a steady state. The complete chamber assembly is shown in Fig. 1. The mucosal and serosal voltage electrodes detected the spontaneous or clamped PD across the gastric mucosa via saline columns and agar saline bridges connected to calomel electrodes (Radiometer K40 1). Current was passed through the same portion of mucosa from Ag-AgCl mucosal electrodes located in the mucosal chamber solution to Ag-AgCl serosal electrodes located in the serosal saline column. PD was recorded continuously except for periodic measurement of current on an automatic null balance servopotentiometer (Electronik 112, Honeywell), which was modified to allow of separation of potential detection and current passage and to allow for a 20-fold amplification of the original