Immunohistochemical study on the neuronal diversity and three-dimensional organization of the mouse entopeduncular nucleus
Immunohistochemical study on the neuronal diversity and three-dimensional organization of the mouse entopeduncular nucleus
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DOI：
10.1016/j.neures.2015.02.006
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发表时间：
2015-05-01
影响因子：
2.9
通讯作者：
Fukuda, Takaichi
中科院分区：
文献类型：
--
作者：
Miyamoto, Yuta;Fukuda, Takaichi
脚内核（EPN）是啮齿类动物基底节的主要输出核团之一。以前的研究将其分为吻侧和尾侧两半，前者含有生长抑素（SUM）免疫反应阳性神经元，后者主要是含有小清蛋白（PV）的神经元。然而，目前还不清楚这种简单的吻尾分割是否合适，以及其他神经元群体存在的可能性仍有待研究。在这项研究中，小鼠EPN的细胞结构进行了化学分析。P物质（SP）的免疫反应性决定的EPN，这是800 μ m长的沿着的吻尾轴的范围。PV阳性神经元集中在尾侧三分之二的这个范围。PV-阴性神经元丰富的吻侧一半，但进一步位于尾侧周围的PV神经元丰富的核心。PV（+）/SOM（-）和PV（-）/SOM（+）神经元分别占EPN神经元的28.6%和45.7%，而其余神经元（25.7%）均无免疫反应。11%的EPN神经元缺乏谷氨酸脱羧酶的免疫反应性，表明它们的非GABA能性质。三维重建显示，富PV/贫SP核被贫PV/富SP壳区包围。因此，假定丘脑靶向PV神经元的数量超过了其他人群，这里显示的区域异质性可能与通过基底神经节的功能不同的途径有关。(C)2015年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
The entopeduncular nucleus (EPN) is one of the major output nuclei of the basal ganglia in rodents. Previous studies have divided it into rostral and caudal halves, with the former containing somatostatin (SUM)-immunoreactive neurons and the latter dominated by parvalbumin (PV)-containing neurons, respectively. However, it is unclear whether this simple rostrocaudal segmentation is appropriate, and the possibility of the existence of other neuronal populations remains to be investigated. In this study the cytoarchitecture of the mouse EPN was analyzed immunohistochemically. Substance P (SP)immunoreactivity determined the extent of the EPN, which was 800 pm-long along the rostrocaudal axis. PV-positive neurons were concentrated in the caudal two-thirds of this range. PV-negative neurons were abundant in the rostral half but were further located caudally around the PV neuron-rich core. PV(+)/SOM(-) and PV(-)/SOM(+) neurons constituted 28.6% and 45.7% of EPN neurons, respectively, whereas the remaining population (25.7%) exhibited neither immunoreactivity. Eleven percent of EPN neurons lacked immunoreactivity for glutamic acid decarboxylase, indicating their non-GABAergic nature. Three-dimensional reconstruction revealed that PV-rich/SP-poor core was surrounded by PV-poor/SP-rich shell region. Therefore, presumptive thalamus-targeting PV neurons are outnumbered by other populations, and the regional heterogeneity shown here might be related to functionally distinct pathways through the basal ganglia. (C) 2015 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.