Synthesis and assembly of wall polymers on regenerating yeast protoplasts.
Synthesis and assembly of wall polymers on regenerating yeast protoplasts.
复制标题
再生酵母原生质体上壁聚合物的合成和组装。
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. Pastor
中科院分区：
文献类型：
--
作者：
R. Sentandreu;E. Herrero;M. Elorza;H. Rico;J. Pastor
几甲壳素和葡聚糖在酿酒酵母和白色念珠菌原生质体上的积累在再生培养基中重悬后不久就开始了，甘露蛋白分子在酿酒酵母中30-60分钟后或在白色念珠菌中经过更长的滞后期后也出现在再生壁上。然而，相当一部分合成的甘露蛋白在sds -丙烯酰胺凝胶中表现出与细胞壁甘露蛋白不同的模式，至少在8小时内仍被释放到生长培养基中。在酿酒酵母的再生过程开始后约30分钟，可以观察到几丁质合成酶的重新合成，而不是葡聚糖合成酶。由白色念珠菌原生质体形成的新生几丁质微原纤维之间的相互作用被钙白或刚果红菌株所改变。在前者存在的情况下，聚合物的微晶格不会形成，原生质体不能正常再生。
Accumulation of chitin and glucan on S. cerevisiae and C. albicans protoplasts begins shortly after resuspension in the regeneration medium, and mannoprotein molecules also appear retained by the regenerating wall after 30-60 minutes in S. cerevisiae or after a longer lag period in C. albicans. Nevertheless, a considerable fraction of the synthesized mannoproteins, which in SDS-acrylamide gels exhibit a different pattern from that of wall manno-proteins of cells, are still released to the growth medium during at least eight hours. De novo synthesis of chitin synthase, but not of glucan synthase, is observed in S. cerevisiae from about 30 minutes after initiation of the regeneration process. The interaction between microfibrils of nascent chitin formed by C. albicans protoplasts is altered by strains as Calcofluor White or Congo Red. In the presence of the former one, no microcrystalline lattice of the polymer is formed and protoplasts do not regenerate correctly.