Characterization of virus-like particles associated with the human faecal and caecal microbiota
Characterization of virus-like particles associated with the human faecal and caecal microbiota
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DOI：
10.1016/j.resmic.2014.10.006
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发表时间：
2014-12-01
影响因子：
2.6
通讯作者：
van Sinderen, Douwe
中科院分区：
文献类型：
--
作者：
Hoyles, Lesley;McCartney, Anne L.;van Sinderen, Douwe
这项工作代表了对与人类粪便和盲肠样本相关的病毒样颗粒 (VLP) 的存在、丰度和多样性的调查。测试和优化了从粪便中回收 VLP 的各种方法，包括为了深入电子显微镜分析、脉冲场凝胶电泳和高效 DNA 回收而成功对此类样品进行下游处理。然后使用从人类盲肠液中获得的样本验证了所开发的 VLP 表征方法的适用性，超出了使用粪便样本的范围。 (C) 2014 年巴斯德研究所。由 Elsevier Masson SAS 出版。版权所有。
This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.