Chronic hypoperfusion alters the content and structure of proteins and lipids of rat brain homogenates: a Fourier transform infrared spectroscopy study
Chronic hypoperfusion alters the content and structure of proteins and lipids of rat brain homogenates: a Fourier transform infrared spectroscopy study
复制标题
DOI：
10.1007/s00249-004-0396-1
复制
发表时间：
2004-03
期刊：
影响因子：
--
通讯作者：
N. Toyran;F. Zorlu;Gizem Dönmez;K. Öğe;F. Severcan
中科院分区：
文献类型：
--
作者：
N. Toyran;F. Zorlu;Gizem Dönmez;K. Öğe;F. Severcan
动静脉畸形（AVM），即大脑中生长的异常血管团块，产生高流量分流，从周围脑组织中窃取血液，而周围脑组织长期灌注不足。低灌注是组织灌注和氧合不足的状况，导致异常的组织代谢。傅里叶变换红外光谱（FTIR）是在这项研究中使用的影响，低灌注对大鼠脑匀浆样品在分子水平上。结果表明，脂质含量增加，蛋白质含量减少，脂质与蛋白质的比例增加，脂质的秩序状态增加，在低灌注脑样品。FTIR结果还表明，由于低灌注，不仅蛋白质合成，而且蛋白质二级结构分布也改变，有利于β-折叠和无规卷曲。这些结果清楚地表明，傅里叶变换红外光谱可以用来提取有价值的信息，在分子水平上，以便有更好地了解低灌注对大鼠脑的影响。
Arteriovenous malformations (AVMs), masses of abnormal blood vessels which grow in the brain, produce high flow shunts that steal blood from surrounding brain tissue, which is chronically hypoperfused. Hypoperfusion is a condition of inadequate tissue perfusion and oxygenation, resulting in abnormal tissue metabolism. Fourier transform infrared (FTIR) spectroscopy is used in this study to investigate the effect of hypoperfusion on homogenized rat brain samples at the molecular level. The results suggest that the lipid content increases, the protein content decreases, the lipid-to-protein ratio increases, and the state of order of the lipids increases in the hypoperfused brain samples. FTIR results also revealed that, owing to hypoperfusion, not only the protein synthesis but also the protein secondary structure profile is altered in favor of β-sheets and random coils. These findings clearly demonstrate that, FTIR spectroscopy can be used to extract valuable information at the molecular level so as to have a better understanding of the effect of hypoperfusion on rat brain.