SAK, A MURINE PROTEIN-SERINE/THREONINE KINASE THAT IS RELATED TO THE DROSOPHILA-POLO KINASE AND INVOLVED IN CELL-PROLIFERATION
SAK, A MURINE PROTEIN-SERINE/THREONINE KINASE THAT IS RELATED TO THE DROSOPHILA-POLO KINASE AND INVOLVED IN CELL-PROLIFERATION
复制标题
DOI：
10.1073/pnas.91.14.6388
复制
发表时间：
1994-07-05
影响因子：
11.1
通讯作者：
DENNIS, JW
中科院分区：
文献类型：
--
作者：
FODE, C;MOTRO, B;DENNIS, JW
我们分离出了编码推定蛋白丝氨酸/苏氨酸激酶的两种亚型的鼠 cDNA，命名为 Sak-a 和 Sak-b，它们的非催化 C 末端不同。 Sak 的激酶结构域与果蝇 polo、酿酒酵母 CDC5 和鼠 Snk 和 Plk 激酶的催化结构域相关，这些激酶是一个已确定在控制细胞增殖中发挥作用的蛋白家族（polo、CDC5）或涉及（Snk、Plk）。对小鼠胚胎和成体组织中 Sak 基因表达的 Northern 和原位 RNA 分析表明，该表达与有丝分裂和减数分裂细胞分裂相关。此外，在胚胎发生过程中，Sak 在呼吸道和嗅粘膜中表达显着。 Sak 表达模式及其与 polo 基因家族的序列同源性表明 Sak 激酶可能在细胞增殖中发挥作用。为了支持这一点，CHO 细胞中 Sak-a 反义片段的表达抑制了细胞生长。
We have isolated murine cDNAs encoding two isoforms of a putative protein-serine/threonine kinase, designated Sak-a and Sak-b, which differ in their noncatalytic C-terminal ends. The kinase domain of Sak is related to the catalyic domains of the Drosophila polo, Saccharomyces cerevisiae CDC5, and murine Snk and Plk kinases, a family of proteins for which a role in controlling cell proliferation has been established (polo, CDC5) or implicated (Snk, Plk). Northern and in situ RNA analyses of Sak gene expression in mouse embryos and adult tissues revealed that expression was associated with mitotic and meiotic cell division. In addition, during embryogenesis, Sak expression was prominent in the respiratory and olfactory mucosa. The pattern of Sak expression and its sequence homology with the polo gene family suggest that the Sak kinase may play a role in cell proliferation. In support of this, cell growth was suppressed by expression of a Sak-a antisense fragment in CHO cells.