Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.
Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.
复制标题
分化胚胎鸡骨骼肌培养物中肌动蛋白信使 RNA 的测定。
DOI：
10.1073/pnas.71.11.4467
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发表时间：
1974
影响因子：
11.1
通讯作者：
D. Yaffe
中科院分区：
文献类型：
--
作者：
B. Paterson;B. Roberts;D. Yaffe
从分化的肌肉培养细胞质聚腺苷酸化信使RNA，在小麦胚芽无细胞系统中孵育时，指导合成的多肽与正宗的鸡骨骼肌肌动蛋白没有区别，判断的流动性十二烷基硫酸钠-聚丙烯酰胺凝胶，胰蛋白酶肽分析，和生物活性。在无细胞系统中肌动蛋白的合成用于测定成纤维细胞、融合前和融合后成肌细胞以及在防止融合的条件下生长的成肌细胞的培养物中可翻译的肌动蛋白mRNA的水平。在所有情况下，在无细胞系统中合成的肌动蛋白多肽的量与提取RNA的培养物中肌动蛋白合成的速率成比例。有人建议，肌动蛋白的合成是由细胞的肌动蛋白mRNA含量的调节，并在细胞质水平的增加，可翻译的肌动蛋白信使RNA介导的细胞融合，而不是由DNA合成的末端轮。
Cytoplasmic polyadenylylated messenger RNA from differentiated muscle cultures, when incubated in a wheat germ cell-free system, directed the synthesis of a polypeptide indistinguishable from authentic chicken skeletal muscle actin, as judged by mobility on sodium dodecyl sulfate-polyacrylamide gels, tryptic peptide analyses, and biological activity. The synthesis of actin in the cell-free system was used to assay levels of translatable actin mRNA in cultures of fibroblasts, pre- and post-fusion myoblasts, and myoblasts grown under conditions that prevent fusion. In all cases the amount of actin polypeptide synthesized in the cell-free system was proportional to the rate of actin synthesis in the cultures from which the RNA was extracted. It is suggested that actin synthesis is regulated by the actin mRNA content of the cell and that an increase in the cytoplasmic level of translatable actin messenger RNA is mediated by cell fusion rather than by the terminal round of DNA synthesis.