Activation and reactivation of Ciona spermatozoa.
Activation and reactivation of Ciona spermatozoa.
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海鞘精子的激活和再激活。
DOI：
10.1002/cm.970020735
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Brokaw,CJ
中科院分区：
文献类型：
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作者：
Brokaw,CJ
这种被囊动物在世界各地的海湾和其他有遮蔽的海洋环境中都有，它是实验工作配子的方便来源，因为它在延伸的管道中储存卵子和精子。与海胆精子相比，琼脂鱼精子鞭毛的实验工作要少得多。一个障碍是脱膜和ATP再激活技术的失败，这些技术对海胆精子非常有效，对乔娜精子也很有效。在过去的几年里，我们逐渐学会了如何修改这些技术，以成功地重新激活脱膜的精子。关键因素是认识到在去膜之前激活精子的运动是必要的。精子运动的激活已经在许多其他物种中进行了研究，Garbers和Kopf[19801]回顾了大量证据，表明在激活过程中普遍涉及camp依赖性磷酸化反应。本文介绍了琼脂精激活和再激活过程的一些特点，为更详细地研究琼脂精鞭毛轴突活性调控机制奠定基础。
The tunicate, Ciona intestinalis, occurs worldwide in bays and other sheltered marine environments, and is a convienient source of gametes for experimental work because it stores eggs and spermatozoa in extended ducts. Experimental work with Ciona sperm flagella has been much less extensive than with sea urchin spermatozoa. One impediment has been the failure of the techniques for demembranation and ATP reactivation, which work very well with sea urchin spermatozoa, to work with Ciona spermatozoa. Over the past few years, we have gradually learned how to modify these techniques to obtain successful reactivation of demembranated Ciona spermatozoa. The key factor has been the realization that it is necessary to activate the motility of the spermatozoa before demembranation. Activation of sperm motility has been studied in many other species, and there is considerable evidence, reviewed by Garbers and Kopf [19801, for a common involvement of a CAMP-dependent phosphorylation reaction in the activation process. This paper describes some characteristics of the activation and reactivation processes in Ciona spermatozoa, as a basis for more detailed studies of the mechanism by which the activity of the flagellar axoneme is regulated in these spermatozoa.