Planar cell polarity governs the alignment of the nasopharyngeal epithelium in mammals
Planar cell polarity governs the alignment of the nasopharyngeal epithelium in mammals
复制标题
平面细胞极性控制哺乳动物鼻咽上皮的排列
DOI：
10.1111/febs.15425
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发表时间：
--
期刊：
影响因子：
5.4
通讯作者：
Dong-dong Ren
中科院分区：
文献类型：
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作者：
Wen-wei Luo;Bin-Jun Chen;Yan-mei Wang;Juan-mei Yang;Xiang Liu;Ya-sheng Yuan;Xi Lin;Fang-lu Chi;Ping Chen;Dong-dong Ren
平面细胞极性（PCP）信号指定上皮细胞的方向，并调节多纤毛上皮细胞的运动纤毛的定向跳动。临床上，纤毛功能的缺陷与鼻咽症状有关。鼻咽上皮的极性知之甚少。在这里，我们证明了PCP在鼻咽上皮。多纤毛细胞（MCCs）均匀排列，其长轴平行于鼻咽（NP）的组织轴。此外，PCP蛋白在相邻细胞之间表现出不对称的定位。运动纤毛在单个细胞和相邻细胞内均沿同一方向均匀排列，表现为MCC的纤毛极性。果蝇PCP基因的哺乳动物同源物Vangl 2的突变导致上皮细胞定向的显著破坏。最后，角蛋白5阳性基底细胞不断补充管腔纤毛细胞;新的动态纤毛细胞也平行于组织轴定向。这些结果表明PCP途径在NP中动态补充的上皮细胞的均匀取向中的作用。
Planar cell polarity (PCP) signalling specifies the orientation of epithelial cells and regulates directional beating of motile cilia of multiciliated epithelial cells. Clinically, defects in cilia function are associated with nasopharyngeal symptoms. The polarity of the nasopharyngeal epithelium is poorly understood. Here, we demonstrated PCP in the nasopharyngeal epithelium. Multiciliated cells (MCCs) were uniformly aligned with their long axis parallel to the tissue axis of the nasopharynx (NP). In addition, PCP proteins exhibited an asymmetrical localisation between adjacent cells. Motile cilia were uniformly aligned in the same direction within both individual cells and neighbouring cells, which manifested as cilial polarity in MCCs. Mutation of Vangl2, a mammalian homologue of the Drosophila PCP gene, resulted in significant disruption of the orientation of epithelial cells. Finally, keratin‐5‐positive basal cells constantly replenished the luminal ciliated cells; the new dynamic ciliated cells were also oriented parallel to the tissue axis. These results indicate a role for the PCP pathway in the uniform orientation of dynamically replenished epithelial cells in the NP.