DNA electrophoresis in microfabricated devices
DNA electrophoresis in microfabricated devices
复制标题
DOI：
10.1103/revmodphys.82.2903
复制
发表时间：
2010-10-06
影响因子：
44.1
通讯作者：
Dorfman, Kevin D.
中科院分区：
文献类型：
--
作者：
Dorfman, Kevin D.
在Viovy对凝胶电泳物理学的评论的结论中，L. Viovy，Rev.Mod.Phys.72，813（2000）]，这篇综述综合了自Kristians和Austin的开创性论文[Nature（伦敦）358，600（1992）]以来出现的微制造和纳米制造装置中DNA电泳的实验数据、理论模型和模拟结果。这些设备的原型版本通过分子量分离DNA，其速度远远上级凝胶电泳。在提供聚合物物理学、电泳和微流体装置制造中的必要背景材料的概述之后，重点是以下三个一般问题：（i）与孤立的后的碰撞，（ii）在后的阵列中的运输，以及（iii）在狭缝-井基序中的熵捕获和过滤。在这里的背景下的长度和时间尺度特征的DNA，设备，和所施加的电场的运输现象进行检查。
Picking up at the conclusion of Viovy's review of the physics of gel electrophoresis [J.-L. Viovy, Rev. Mod. Phys. 72, 813 (2000)], this review synthesizes the experimental data, theoretical models, and simulation results for DNA electrophoresis in microfabricated and nanofabricated devices appearing since the seminal paper by Volkmuth and Austin [Nature (London) 358, 600 (1992)]. Prototype versions of these devices separate DNA by molecular weight at a rate far superior to gel electrophoresis. After providing an overview of the requisite background material in polymer physics, electrophoresis, and microfluidic device fabrication, the focus is on the following three generic problems: (i) collision with an isolated post, (ii) transport in an array of posts, and (iii) entropic trapping and filtration in the slit-well motif. The transport phenomena are examined here in the context of the length and time scales characterizing the DNA, the device, and the applied electric field.