Exaggerated inflammatory responses mediated by Burkholderia cenocepacia in human macrophages derived from Cystic fibrosis patients
Exaggerated inflammatory responses mediated by Burkholderia cenocepacia in human macrophages derived from Cystic fibrosis patients
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DOI：
10.1016/j.bbrc.2012.06.066
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发表时间：
2012-07-27
影响因子：
3.1
通讯作者：
Amer, Amal O.
中科院分区：
文献类型：
--
作者：
Kopp, Benjamin T.;Abdulrahman, Basant A.;Amer, Amal O.
囊性纤维化（CF）伴随着由耐药性新洋葱伯克霍尔德氏菌恶化的炎症加剧。人CF巨噬细胞对B的反应。在文献中，新洋葱虫的特征很差并且是可变的。因此，我们检测了人巨噬细胞对流行性B的反应。Cenocepacia J2315菌株，以鉴定新的抗炎靶标。从23名CF和27名非CF供体获得外周血单核细胞衍生的巨噬细胞。用B.新洋葱头J2315中的细胞，并分析细胞因子、细胞毒性和显微镜检查。与非CF对照相比，CF巨噬细胞表现出IL-1 β、IL-10、MCP-1和IFN-γ产生的显著增加。与对照组和其他CF患者相比，接受泼尼松治疗的CF患者表现出全面减少的细胞因子。CF巨噬细胞还显示出增加的细菌负荷和细胞死亡。总之，CF巨噬细胞在B期间表现出过度的IL-1 β、IL-10、MCP-1和IFN-γ产生和细胞死亡。新洋葱感染皮质类固醇治疗急性抑制细胞因子反应。(C)2012 Elsevier Inc. All rights reserved.
Cystic fibrosis (CF) is accompanied with heightened inflammation worsened by drug resistant Burkholderia cenocepacia. Human CF macrophage responses to B. cenocepacia are poorly characterized and variable in the literature. Therefore, we examined human macrophage responses to the epidemic B. cenocepacia J2315 strain in order to identify novel anti-inflammatory targets. Peripheral blood monocyte derived macrophages were obtained from 23 CF and 27 non-CF donors. Macrophages were infected with B. cenocepacia J2315 and analyzed for cytokines, cytotoxicity, and microscopy. CF macrophages demonstrated significant increases in IL-1 beta, IL-10, MCP-1, and IFN-gamma production in comparison to non-CF controls. CF patients on prednisone exhibited globally diminished cytokines compared to controls and other CF patients. CF macrophages also displayed increased bacterial burden and cell death. In conclusion, CF macrophages demonstrate exaggerated IL-1 beta, IL-10, MCP-1, and IFN-gamma production and cell death during B. cenocepacia infection. Treatment with corticosteroids acutely suppressed cytokine responses. (C) 2012 Elsevier Inc. All rights reserved.