Effects of allotype specific anti-IgD on the immune responses of homozygous mice.
Effects of allotype specific anti-IgD on the immune responses of homozygous mice.
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同种异型特异性抗 IgD 对纯合小鼠免疫反应的影响。
DOI：
10.1111/j.1749-6632.1982.tb25683.x
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发表时间：
1982
影响因子：
5.2
通讯作者：
Jacobson,EB
中科院分区：
文献类型：
--
作者：
Thorbecke,GJ;Baine,Y;Xue,B;Chen,YW;Pernis,B;Siskind,GW;Jacobson,EB
在之前对出生后就接受抗 IgD 治疗的小鼠的研究中，我们发现静脉注射（iv 1）三硝基苯基化流产布鲁氏菌 (TNP-BA) 的初级或次级反应没有受到抑制。考虑到此类抗 IgD 治疗的小鼠脾脏中存在的 Ig 携带细胞数量较少，这被认为是令人惊讶的。还发现对包括 TNP-hernocyanin (TNP-KLH] 或 TNP-ficoll 在内的其他抗原的初级反应尽管几乎不存在 IgD+ B 细胞，但 TNP-ficoll 的正常免疫反应的存在（通常归因于 Lyb5+ 细胞）促使我们与 R. Skelly 和 A. Ahmed（默克研究所，拉威，新泽西州）合作确定 Lyb5+ 细胞是否存在于脾脏中发现的约 2-10% Ig+ 细胞中。抗 IgD 处理的小鼠中发现了相对较高比例的 Lyb5+ 细胞？此外，对抗 IgD 处理的小鼠的淋巴组织进行的组织学观察显示，尽管通过免疫过氧化物酶 (PAP) 在正常小鼠的冠状中缺乏通常存在于每个生发中心的致密淋巴细胞冠，但仍存在具有正常有丝分裂率、母细胞含量和花生亲和力的生发中心。其他人也报告了类似的结果。'-'结论是，再循环 IgD+ B 细胞的缺乏并不影响对静脉注射抗原的反应，并且正常小鼠和抗 IgD 抑制小鼠之间 B 细胞成熟和/或增殖的差异主要是定量的，可能是因为 IgD+ B 细胞的缺乏（至少在脾脏中）通过 IgM+ 的增强得到了补偿。 IgD-B 细胞。
In previous studies' on mice treated with anti-IgD from birth we found no suppression of primary or secondary responses to trinitrophenylated B. abortus (TNP-BA) injected intravenously (iv 1. This was considered surprising in view of the low numbers of Ig-bearing cells present in the spleens of such anti-IgD treated mice. Primary responses to other antigens including TNP-hernocyanin (TNP-KLH] or TNP-ficoll were also found to be, if anything, enhanced rather than suppressed in spite of the virtual absence of IgD+ B-cells (TABLE 1). The presence of normal immune responses to TNP-ficoll, a response usually attributed to Lyb5+ cells? prompted us to determine, in collaboration with R. Skelly and A. Ahmed (Merck Institute, Rahway, NJ], whether Lyb5+ cells were present among the approximately 2-10% Ig+ cells found in the spleen of anti-IgD treated mice. A relatively high percentage of Lyb5+ cells was found? In addition, histological observations of the lymphoid tissues of the anti-IgD treated mice showed the presence of germinal centers which had a normal mitotic rate, blast cell content, and affinity for peanut even though the dense lymphocyte coronas, normally present next to each germinal center, were lacking. IgD+ cells could be detected in the coronas of normal mice by immunoperoxidase (PAP) staining,'.'~~ but were absent in the suppressed mice. Similar findings have been reported by others.'-'It was concluded that the deficiency in recirculating IgD+ B-cells did not affect responses to intravenously injected antigens, and that the difference in B-cell maturation and/or proliferation between normal and anti-IgD suppressed mice was primarily quantitative, possibly because the deficiency in IgD+ B-cells, at least in the spleen, was compensated for by enhanced representation of IgM+ IgD-B-cells.