EFFICIENT SYNTHESIS OF OLIGORIBONUCLEOTIDE AND ITS PHOSPHOROTHIOATE ANALOG USING H-PHOSPHONATE APPROACH
EFFICIENT SYNTHESIS OF OLIGORIBONUCLEOTIDE AND ITS PHOSPHOROTHIOATE ANALOG USING H-PHOSPHONATE APPROACH
复制标题
DOI：
10.1016/s0040-4039(00)97293-9
复制
发表时间：
1990-01-01
影响因子：
1.8
通讯作者：
TANG, JY
中科院分区：
文献类型：
--
作者：
AGRAWAL, S;TANG, JY
利用二甲氧基三苯甲基（DMTr）和叔丁基二甲基甲硅烷基（t-BDMS）对核糖核苷单体进行5 ′-保护和2 ′-保护，并通过H-膦酸酯偶联，高效固相合成了寡核糖核苷酸及其硫代磷酸酯类似物。 合成循环经过优化，在每个偶联步骤中仅使用8-10倍过量的单体，导致平均偶联产率为97%。
Efficient solid phase synthesis of oligoribonucleotide and its phosphorothioate analogue is described that utilizes the dimethoxytrityl (DMTr) for 5'-protection and t-butyldimethylsilyl (t-BDMS) group for 2'-protection of ribonucleoside monomers and the H-phosphonate coupling procedure. The synthetic cycles have been optimised to use only 8-10 fold excess of monomer at each coupling step, leading to an average coupling yield of 97%.