Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats
Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats
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DOI：
10.1023/a:1003516620466
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发表时间：
1999-01-01
期刊：
影响因子：
1.9
通讯作者：
Hirano, H
中科院分区：
文献类型：
--
作者：
Nakamura, H;Inazu, A;Hirano, H
采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法对131个日本野菱小麦品种的种子贮藏蛋白进行了分离，以确定控制高分子量麦谷蛋白亚基的3个位点的等位基因组成。在Glu-A1位点鉴定出3个等位基因，在Glu-B1位点鉴定出6个等位基因，在Glu-D1位点鉴定出5个等位基因。24个不同的，主要的谷蛋白高分子量亚基，每个含有3至5个亚基和17个不同的谷蛋白亚基模式观察到19个亚基在131个日本野灵品种。通过亚基迁移率与先前在六倍体小麦中发现的亚基迁移率的比较，鉴定出14个等位基因。日本野菱小麦品种在Glu-1位点上表现出与中国及其他国家普通小麦不同的高分子量麦谷蛋白亚基等位变异模式。
Seed storage proteins of 131 Japanese Norin wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine allelic make-up in varieties at each of three loci that control high-molecular-weight (HMW) glutenin subunits. Three alleles were identified at the Glu-A1 locus, six at the Glu-B1 locus and five at the Glu-D1 locus. Twenty-four different, major glutenin HMW subunits were identified and each contained three to five subunits and seventeen different glutenin subunit patterns were observed for 19 subunits in the 131 Japanese Norin varieties. Fourteen alleles were identified by comparison of subunit mobility with that previously found in hexaploid wheat. Japanese Norin varieties showed a specific pattern of allelic variation in glutenin HMW subunits, different from that of Chinese and other country common wheats in allelic frequency at Glu-1 loci.