Comparison of efficiency between two artificial insemination methods using frozen-thawed semen in domestic cat (Felis catus) Artificial insemination in domestic cats
Comparison of efficiency between two artificial insemination methods using frozen-thawed semen in domestic cat (Felis catus) Artificial insemination in domestic cats
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DOI：
10.1016/j.anireprosci.2008.10.008
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发表时间：
2009-09-01
影响因子：
2.2
通讯作者：
Lopes, Maria Denise
中科院分区：
文献类型：
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作者：
Silva Balbin Villaverde, Ana Izabel;Melo, Cely Marini;Lopes, Maria Denise
本研究的目的是比较家猫使用冻融精子进行阴道内人工授精 (IVAI) 与宫内人工授精 (IUAI) 的效率。使用人工阴道从两只公猫身上收集精液，并评估样本的活力（计算机辅助精子分析（CASA））、精子形态和质膜完整性。用 TRIS/OEP/YOLK（4% 甘油）稀释后，将精子样品装入 0.25 mL 细管（25 x 106 活动精子/细管）中，在 5°C 下孵育 20 分钟，并在液氮 (LNZ) 蒸气中冷冻保存 15 分钟，然后浸入液氮中。对于每个 AI，将来自同一雄性的四根细管解冻（46 摄氏度下 12 秒）并以 250 x g 离心 8 分钟以沉淀精子。弃去上清液，并用剩余液体（大约100μL）重悬精子沉淀，并如上所述进行分析。皇后接受单次肌内注射 100 IU eCG 以诱导卵泡发育。在施用 ECG 后 82-84 小时，通过肌内注射 100 IU hCG 诱导最终卵母细胞成熟和排卵。施用 hCG 30 小时后，使用来自单个雄性的解冻精子在子宫内（n = 8 只蜂王）或阴道内（n = 8 只蜂王）对雌性进行授精。尽管在两只猫的精子样本中都观察到精子活力、顶体和质膜完整性明显下降，但使用宫内 AI 方法时的妊娠率达到 75%，而阴道内授精的妊娠率为 0%。 (C) 2008 年由 Elsevier B.V. 出版
The aim of this study was to compare the efficiency of the intravaginal (IVAI) vs. intrauterine artificial insemination (IUAI) using frozen-thawed sperm in the domestic cat. Semen was collected from two tom cats using an artificial vagina and samples were assessed for motility (computer-assisted sperm analysis (CASA)), sperm morphology and plasma membrane integrity. After dilution with TRIS/OEP/YOLK (4% of glycerol), sperm samples were loaded into 0.25 mL straws (25 x 106 motile sperm/straw), incubated at 5 degrees C for 20 min and cryopreserved over liquid nitrogen (LNZ) vapor for 15 min and then immersed in LN2. For each AI, four straws from the same male were thawed (12 s at 46 degrees C) and centrifuged at 250 x g for 8 min to pellet the sperm. The supernatant was discarded and sperm pellet resuspended with the remaining liquid, approximately 100 mu L, and analyzed as described above. Queens were treated with a single im injection of 100 IU eCG to induce ovarian follicular development. Final oocyte maturation and ovulation was induced with 100 IU hCG given im at 82-84 h after eCG administration. Thirty hours after hCG administration, females were inseminated either intrauterine (n=8 queens) or intravaginally (n=8 queens), using thawed sperm from a single male. Although a pronounced decrease in sperm motility, acrosome and plasma membrane integrity was observed in sperm samples from both cats, a pregnancy rate of 75% was achieved when using the intrauterine AI method compared with 0% pregnancy when inseminated intravaginally. (C) 2008 Published by Elsevier B.V.