Two Drosophila melanogaster tropomyosin genes: structural and functional aspects
Two Drosophila melanogaster tropomyosin genes: structural and functional aspects
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两个果蝇原肌球蛋白基因：结构和功能方面
DOI：
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发表时间：
1986
影响因子：
5.3
通讯作者：
E. A. Fyrberg
中科院分区：
文献类型：
--
作者：
C. C. Karlik;E. A. Fyrberg
我们比较了果蝇两个黑腹果蝇原肌球蛋白基因的结构和功能。最引人注目的结构性方面是它们的规模差距。基因2的第1~257密码子占833个核苷酸，只有1个内含子，而基因1的对应区域有17.5kb的碱基，被8个内含子打断。基因1的内含子-外显子排列反映了原肌球蛋白通过42和49个残基重复的进化扩展，这些重复可能是肌动蛋白结合域。在功能上，基因1比基因2复杂得多；它在肌肉和非肌肉细胞系中都很活跃，至少有五个可变外显子，并至少指定了五种发育调节的亚型。其中两个仅在飞行肌肉中积累的亚型是史无前例的融合蛋白，其中原肌球蛋白序列连接到一个羧基末端的富含脯氨酸的结构域。
We compared the structure and function of the two Drosophila melanogaster tropomyosin genes. The most striking structural aspect was their size disparity. Codons 1 through 257 of gene 2 occupied 833 nucleotides and contained only one intron, whereas the corresponding region of gene 1 occupied 17.5 kilobases and was interrupted by eight introns. The intron-exon arrangement of gene 1 reflected evolutionary expansion of tropomyosin via 42- and 49-residue duplications, which are probably actin-binding domains. Functionally, gene 1 was considerably more complex than gene 2; it was active in both muscle and nonmuscle cell lineages, had at least five variable exons, and specified a minimum of five developmentally regulated isoforms. Two of these isoforms, which accumulated only in flight muscles, were unprecedented fusion proteins in which the tropomyosin sequence was joined to a carboxy-terminal proline-rich domain.
果蝇中与两个肌肉原肌球蛋白基因连锁的细胞质原肌球蛋白基因的鉴定。
DOI：
10.1073/pnas.80.23.7123
发表时间：
1983
影响因子：
11.1
作者：
Bautch,VL;Storti,RV
通讯作者：
Storti,RV
从正常和转化的大鼠培养细胞中分离的微丝中原肌球蛋白的差异表达形式。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Matsumura,F;Lin,JJ;Yamashiro-Matsumura,S;Thomas,GP;Topp,WC
通讯作者：
Topp,WC