Intake of heated leaf extract of Coriandrum sativum contributes to resistance to oxidative stress via decreases in heavy metal concentrations in the kidney
Intake of heated leaf extract of Coriandrum sativum contributes to resistance to oxidative stress via decreases in heavy metal concentrations in the kidney
复制标题
摄入加热的芫荽叶提取物有助于通过降低肾脏中的重金属浓度来抵抗氧化应激
DOI：
10.1007/s11130-019-00720-2
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Azusa Takeuchi and Atsushi Takeda
中科院分区：
文献类型：
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作者：
Ryusuke Nishio;Hanuna Tamano;Hiroki Morioka;Azusa Takeuchi and Atsushi Takeda
芫荽（Coriandrum sativum）是伞形科（Apiaceae）的一年生草本植物，一直被用作传统药物。在这里，我们研究是否加热香菜叶提取物降低组织中的重金属浓度。给雄性ddY小鼠饮用含有0.25%加热的芫荽叶提取物的饮用水8周。摄入8周后，测量肝脏和肾脏中锌、铁、铜、砷和镉的浓度。香菜的摄入量并没有改变肝脏中所有重金属的浓度，但降低了肾脏中铁，砷和镉的浓度。由于重金属可以诱导氧化应激，香菜摄入对过氧化氢诱导的氧化应激的影响进行了比较之间的切片从肾脏和肝脏。将切片浸入含有100 μM过氧化氢和氨基苯基荧光素（APF）（用于检测活性氧（ROS）的探针）的林格溶液中。对照组肾组织APF荧光明显增强，而芫荽组肾组织APF荧光增强被完全阻断。与此相反，APF荧光也显着增加，在控制肝脏切片，而增加没有被香菜摄入量。目前的研究表明，香菜叶提取物的摄入有助于肾脏对氧化应激的强大抵抗力，可能是通过降低重金属浓度。砷浓度降低到检测限可能是耐药性的主要因素。
Coriandrum sativum(coriander) is an annual herb of the Apiaceae family and has been used as a traditional remedy. Here we examined whether heated leaf extract of coriander decreases the concentrations of heavy metals in tissues. Male ddY mice were given a drinking water containing 0.25% of heated leaf extract of coriander for 8 weeks. Eight weeks after the intake, the concentrations of zinc, iron, copper, arsenic, and cadmium were measured in the liver and kidney. The intake of coriander did not modify the concentrations of all heavy metals tested in the liver, but decreased the concentrations of iron, arsenic, and cadmium in the kidney. Because heavy metals can induce oxidative stress, the effect of coriander intake on hydrogen peroxide-induced oxidative stress was compared between slices from the kidney and liver. The slices were immersed in Ringer solution containing 100 μM hydrogen peroxide and aminophenyl fluorescein (APF), a probe for detecting reactive oxygen species (ROS). APF fluorescence was markedly increased in the control kidney slices, while the increase was completely blocked in kidney slices from coriander intake group. In contrast, APF fluorescence was also markedly increased in the control liver slices, while the increase was not blocked by coriander intake. The present study indicates that intake of coriander leaf extract contributes to powerful resistance to oxidative stress in the kidney, probably via decreased concentrations in heavy metals. It is likely that decrease in arsenic concentration to the detection limit is a major factor for the resistance.