DETERMINATION OF THE ORIGIN AND NATURE OF BRAIN MACROPHAGES AND MICROGLIAL CELLS IN MOUSE CENTRAL-NERVOUS-SYSTEM, USING NONRADIOACTIVE INSITU HYBRIDIZATION AND IMMUNOPEROXIDASE TECHNIQUES
DETERMINATION OF THE ORIGIN AND NATURE OF BRAIN MACROPHAGES AND MICROGLIAL CELLS IN MOUSE CENTRAL-NERVOUS-SYSTEM, USING NONRADIOACTIVE INSITU HYBRIDIZATION AND IMMUNOPEROXIDASE TECHNIQUES
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DOI：
10.1002/glia.440060408
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发表时间：
1992-01-01
期刊：
影响因子：
6.2
通讯作者：
DIJKSTRA, CD
中科院分区：
文献类型：
--
作者：
DEGROOT, CJA;HUPPES, W;DIJKSTRA, CD
研究了脑巨噬细胞和小胶质细胞在小鼠中枢神经系统（CNS）中的来源和性质。首先，利用免疫组织化学方法研究了不同单克隆抗体（mab） MOMA-1、mac -1- α和F4/80（针对单核吞噬细胞系统细胞）识别的决定因子在发育和成年小鼠大脑中的表达和定位。为了明确脑巨噬细胞和小胶质细胞的来源，我们使用噬菌体lambda转基因小鼠作为供体，对不同年龄的受体小鼠进行骨髓移植。在个体发育过程中，大量的MOMA-1-、mac -1- α -和f4 /80阳性的血单核细胞源性脑巨噬细胞（变形虫小胶质细胞）浸润中枢神经系统实质。这些巨噬细胞在出生后第7天逐渐从脑实质中消失（P7）。从P17开始，在脑实质内检测到mac -1- α -和f4 /80阳性细胞，具有静息小胶质细胞的形态。在发育中的大脑中未观察到脑巨噬细胞和“静息”小胶质细胞之间的过渡形式。结合非放射性原位杂交和免疫组织化学发现许多moma -1阳性的骨髓源性脑巨噬细胞位于脑膜、脑室，偶尔也位于血管壁。这些结果表明，脑巨噬细胞来源于骨髓。在大脑中检测到许多“静止”的小胶质细胞，主要在白质中。这些细胞中大约有10%显示出转基因信号。这个结果。表明大多数“静止”的小胶质细胞是局部的，可能是神经外胚层的。
The origin and nature of brain macrophages and microglial cells in the mouse central nervous system (CNS) were investigated. First, the expression and localization of determinants recognized by the different monoclonal antibodies (mAbs) MOMA-1, Mac-1-alpha, and F4/80 (raised against cells of the mononuclear phagocyte system) were immunohistochemically studied in the developing and adult mouse brain. In order to clarify the origin of brain macrophages and microglial cells, we used bacteriophage lambda transgenic mice as donors for bone marrow transplantations in recipient mice of different ages.During ontogeny, numerous MOMA-1-, Mac-1-alpha-, and F4/80-positive blood monocyte-derived brain macrophages (amoeboid microglia) infiltrated the CNS parenchyma. These brain macrophages gradually disappeared from the brain parenchyma at postnatal day 7 (P7). From P17 on, Mac-1-alpha- and F4/80-positive cells were detected within the brain parenchyma with the morphology of resting microglial cells. Transitional forms between brain macrophages and "resting" microglia were not observed in the developing brain.Combined non-radioactive in situ hybridization and immunohistochemistry revealed many MOMA-1-positive bone marrow-derived brain macrophages that were located in the leptomeninges, the ventricles, and occasionally the blood vessel walls. These results show that brain macrophages are of bone marrow origin. Many "resting" microglial cells were detected in the brain, mainly in the white matter. It appeared that about 10% of these cells displayed the transgenic signal. This result.indicates that the majority of "resting" microglial cells are of local, presumably neuroectodermal, origin.