Atypical BSE in Germany - Proof of transmissibility and biochemical characterization
Atypical BSE in Germany - Proof of transmissibility and biochemical characterization
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DOI：
10.1016/j.vetmic.2006.06.016
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发表时间：
2006-10-31
影响因子：
3.3
通讯作者：
Groschup, Martin H.
中科院分区：
文献类型：
--
作者：
Buschmann, A.;Gretzschel, A.;Groschup, Martin H.
密集的主动监测在老年牛中发现了两例非典型的德国疯牛病病例，这两例病例与最近在法国(指定为H型)和意大利(指定为L型或基地)描述的两种不同的非典型疯牛病表型相似。H型具有明显较大的分子大小，但常规糖型的蛋白酶K处理异常蛋白(PrPSc)，而L型PrPSc仅具有较小的分子尺寸和明显不同的糖型。在这篇文章中，我们描述了两例德国非典型疯牛病病例成功地传播到过度表达牛PrPC的转基因小鼠。在挑战L类型的小鼠后，这些小鼠患上疯牛病的潜伏期比迄今为止使用这些小鼠传播的任何经典的疯牛病都要短得多。相反，当这些小鼠被H型病毒攻击时，潜伏期明显延长。在这些小鼠大脑中积累的PrPSc与用于传播的非典型疯牛病类型相同。这些非典型病例表明牛中可能存在散发的疯牛病病例。因此，英国的BSE疫情也可能是由一例散发的BSE病例的种内传播引发的，这是可行的。(C)2006爱思唯尔B.V.保留所有权利。
Intensive active surveillance has uncovered two atypical German BSE cases in older cattle which resemble the two different atypical BSE phenotypes that have recently been described in France (designated H-type) and Italy (designated L-type or BASE). The H-type is characterized by a significantly higher molecular size, but a conventional glycopattern of the proteinase K treated abnormal prion protein (PrPSc), while the L-type PrPSc has only a slightly lower molecular size and a distinctly different glycopattern. In this paper we describe the successful transmission of both German atypical BSE cases to transgenic mice overexpressing bovine PrPC. Upon challenge with the L-type, these mice developed BSE after a substantially shorter incubation period than any classical BSE transmission using these mice to date. In contrast, the incubation period was distinctly prolonged when these mice were challenged with the H-type. PrPSc accumulated in the brains of these mice were of the same atypical BSE type that had been used for the transmission. These atypical cases suggest the possible existence of sporadic BSE cases in bovines. It is thus feasible that the BSE epidemic in the UK could have also been initiated by an intraspecies transmission from a sporadic BSE case. (c) 2006 Elsevier B.V. All rights reserved.