Rhodiola rosea ability to enrich cellular antioxidant defences of cultured human keratinocytes
Rhodiola rosea ability to enrich cellular antioxidant defences of cultured human keratinocytes
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DOI：
10.1007/s00403-009-0985-z
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发表时间：
2010-04-01
影响因子：
3
通讯作者：
Dacha, Marina
中科院分区：
文献类型：
--
作者：
Calcabrini, Cinzia;De Bellis, Roberta;Dacha, Marina
角质形成细胞是暴露于氧化应激的细胞，但通常具有良好的抗氧化反应。然而，长期以来，人们一直认为外源性抗氧化剂可以在减少与此类氧化剂相关的不良皮肤反应方面发挥有用的作用。由于其组成成分具有重要的活性，且各成分的相互分布，本研究将红景天根茎的提取作为一个整体进行研究。我们测量了提取物对暴露于不同氧化损伤(Fe（II)/抗坏血酸、Fe(II)/H2O2和叔丁基过氧化氢）的培养人角质形成细胞（NCTC 2544）中还原型谷胱甘肽水平、甘油醛-3-磷酸脱氢酶活性和硫代巴比托酸活性物质水平的保护作用。我们还研究了玫瑰红提取物对细胞内活性氧产生和抗氧化酶（过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶）活性的影响。此外，我们已经证明，玫瑰红提取物能够以时间和剂量依赖的方式增加反质膜氧化还原酶活性，作为细胞内氧化还原状态的间接评价，这种效果在小浓度和长时间的提取物中已经很明显。结果表明，NCTC 2544能较好地抵抗几种氧化损伤，表明该植物复合物具有良好的抗氧化活性。
Keratinocytes are cells strongly exposed to oxidative stress, but normally good equipped for antioxidant responses. However, it has long been suggested that exogenous antioxidants could play a useful role in minimizing the adverse skin responses associated with such oxidant species. In this work it was paid attention to the extract of Rhodiola rosea L. roots by using the phytocomplex as a whole because of the important activity of its composition and mutual distribution of its components. We have measured the protection afforded by the extract to reduced glutathione levels, glyceraldehyde-3-phosphate dehydrogenase activity, and thiobarbituric acid reactive substances levels in cultured human keratinocytes (NCTC 2544) exposed to different oxidative insults: Fe(II)/ascorbate, Fe(II)/H2O2, and tert-butyl-hydroperoxide. We also have investigated the influence of the R. rosea extract on the production of intracellular reactive oxygen species and on the activity of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase). Furthermore, we have demonstrated that R. rosea extract was able to increase in a time- and dose-dependent manner the activity of the trans plasma membrane oxido reductase activity as an indirect evaluation of the intracellular redox status and this effect was already evident with small concentration of the extract and in a long time. As a result, NCTC 2544 are able to better counteract to several oxidative insults if incubated with R. rosea extract demonstrating a very good antioxidant activity of this phytocomplex.