Analysis of the Intestinal Microbiome of a Recovered Clostridium difficile Patient after Fecal Transplantation
Analysis of the Intestinal Microbiome of a Recovered Clostridium difficile Patient after Fecal Transplantation
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DOI：
10.1159/000355955
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发表时间：
2013-01-01
期刊：
影响因子：
3.2
通讯作者：
Moelling, Karin
中科院分区：
文献类型：
--
作者：
Broecker, Felix;Kube, Michael;Moelling, Karin
背景：抗生素破坏肠道微生物群后的艰难梭菌感染可能是致命的。粪便微生物群移植（FMT）是复发性艰难梭菌相关疾病（CDAD）的一种有前途的治疗选择。在这里，我们提出了一个复发性CDAD患者接受FMT，导致完全恢复了什么，现在已经3年。我们对粪便样本进行了宏基因组测序，以评估FMT后是否存在艰难梭菌感染和肠道微生物群变化的迹象。方法：取供、受者粪便DNA进行Illumina测序。将获得的读段集组装成连续序列，并预测开放阅读框。推导出的蛋白质进行分类分配。结果：FMT后，我们在供者和受者的肠道中检测到复杂的和表面上健康的微生物组，但没有C。艰难的殖民结论：宏基因组分析被证明适用于分析FMT后的肠道微生物组。对我们的评估程序和数据管理的讨论可能有助于未来的研究。我们证明了用来自供体和受体的嵌合组合物恢复健康和多样化的肠道微生物组，以及持久的C.很难该手术简单，便宜，没有副作用，并且稳定超过3年。(C)2013 S. Karger AG，巴塞尔
Background: Clostridium difficile infections upon antibiotic disruption of the gut microbiota are potentially lethal. Fecal microbiota transplantation (FMT) is a promising treatment option for recurrent C difficile-associated disease (CDAD). Here, we present a patient with recurrent CDAD that received FMT, leading to full recovery for what has now been 3 years. We performed metagenomic sequencing on stool samples to assess if there are indications for recolonization with C difficile and changes in the gut microbiota after FMT. Methods: DNA from the stool of the donor and recipient was subjected to illumina sequencing. Obtained read sets were assembled to contiguous sequences and open reading frames were predicted. Deduced proteins were taxonomically assigned. Results: We detected complex and apparently healthy nnicrobiomes in the donor's and recipient's intestines after FMT, but no indications for C. difficile colonization. Conclusions: Metagenomic analysis proved suitable to analyze the intestinal microbiome after FMT. Discussion of our evaluation procedure and data management may be helpful for future studies. We demonstrated restoration of a healthy and diverse gut microbiome with chimeric composition from donor and recipient, and long-lasting Clearance of C. difficile. The procedure is simple, cheap, caused no side effects, and was stable over 3 years. (C) 2013 S. Karger AG, Basel