Surface reaction of Leishmania
Surface reaction of Leishmania
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利什曼原虫的表面反应
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
L. Schnur
中科院分区：
文献类型：
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作者：
R. Jacobson;G. Slutzky;C. Greenblatt;L. Schnur
用一组凝集素测定了12株利什曼原虫原鞭毛菌的表面糖残基，并根据其排泄因子（EF）血清型进行了分组。抑制糖用于测定凝集素的特异性。血清型A与大豆凝集素反应，表明对n -乙酰- d -半乳糖胺具有特异性，而B血清型缺乏这种决定因素。A2亚血清型的分化是由于其对α- d -葡萄糖和α- d -甘露糖特异性的刀豆蛋白A具有更强的反应性。蓖麻凝集素对β- d -半乳糖和n -乙酰-半乳糖具有特异性，对A1和B1亚血清型反应最强。花生凝集素对半乳糖具有特异性，可区分A2B2和A3B2混合亚血清型。小麦胚芽凝集素和植物血凝素- p均不能凝集任何被试菌株的原鞭毛菌。
A panel of lectins was used to determine surface sugar residues on promastigotes of twelve isolates of Leishmania, grouped on the basis of their excreted factor (EF) serotype. Inhibiting sugars were used to determine the specificity of the lectins. Serotype A reacted with soybean agglutinin, indicating a specificity for N-acetyl-D-galactosamine, while B serotypes lacked this determinant. Subserotype A2 was differentiated by its greater reactivity to Concanavalin A, specific for α-D-glucose and α-D-mannose. Ricinus agglutinin, specific for β-D-galactose and N-acetyl- galactosamine, gave the strongest reactions with the A1 and B1 subserotypes. The mixed sub-serotypes, A2B2 and A3B2, were differentiated by peanut agglutinin, which is specific for galactose. Wheat germ agglutinin and Phytohaemagglutinin-P did not agglutinate the promastigotes of any of the strains tested.