Gain of deleterious function causes an autoimmune response and Bateson–Dobzhansky–Muller incompatibility in rice
Gain of deleterious function causes an autoimmune response and Bateson–Dobzhansky–Muller incompatibility in rice
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DOI：
10.1007/s00438-010-0514-y
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发表时间：
2010-02
影响因子：
3.1
通讯作者：
E. Yamamoto;T. Takashi;Y. Morinaka;Shaoyang Lin;Jianzhon Wu;T. Matsumoto;H. Kitano;M. Matsuoka-
中科院分区：
文献类型：
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作者：
E. Yamamoto;T. Takashi;Y. Morinaka;Shaoyang Lin;Jianzhon Wu;T. Matsumoto;H. Kitano;M. Matsuoka-
生殖隔离在物种形成中起着重要作用，它限制了基因流动，加速了原杂交种群间的遗传分化。在水稻中，杂交失败是种内和种间杂交中常见的生殖隔离。这是一种合子后生殖隔离，在F2代及以后世代表现为不育和虚弱。本研究以粳稻品种越光（Koshihikari）和粳稻品种羽崎（Habataki）为材料，研究了hbd 2和hbd 3两个隐性基因导致杂种败育的生理和分子基础。对hbd 2进行精细定位，鉴定出致病基因为酪蛋白激酶I（CKI 1）。进一步分析表明，bd 2-CKI 1等位基因通过一个氨基酸的改变获得了其导致虚弱表型的有害功能。另一个基因hbd 3定位在NBS-LRR基因簇区域。它是最常见的一类R基因，触发免疫信号以响应病原体攻击。病原体反应标记基因的表达分析表明，在这种杂交故障的弱点表型可以归因于自身免疫反应。到目前为止，这是第一个将自身免疫反应与水稻合子后隔离联系起来的证据。这一发现为理解植物合子后隔离的分子和进化机制提供了新的见解。
Reproductive isolation plays an important role in speciation as it restricts gene flow and accelerates genetic divergence between formerly interbreeding population. In rice, hybrid breakdown is a common reproductive isolation observed in both intra and inter-specific crosses. It is a type of post-zygotic reproductive isolation in which sterility and weakness are manifested in the F2and later generations. In this study, the physiological and molecular basis of hybrid breakdown caused by two recessive genes,hbd2andhbd3, in a cross betweenjaponicavariety, Koshihikari, andindicavariety, Habataki, were investigated. Fine mapping ofhbd2resulted in the identification of the causal gene ascasein kinase I(CKI1). Further analysis revealed thathbd2-CKI1 allele gains its deleterious function that causes the weakness phenotype by a change of one amino acid. As for the other gene,hbd3was mapped to theNBS-LRRgene cluster region. It is the most common class ofR-gene that triggers the immune signal in response to pathogen attack. Expression analysis of pathogen response marker genes suggested that weakness phenotype in this hybrid breakdown can be attributed to an autoimmune response. So far, this is the first evidence linking autoimmune response to post-zygotic isolation in rice. This finding provides a new insight in understanding the molecular and evolutionary mechanisms establishing post-zygotic isolation in plants.